Effects of silver nanowire length and exposure route on cytotoxicity to earthworms

To accurately evaluate the environmental toxicity of silver nanowires (AgNWs), it is necessary to characterize how the cytotoxicity of these nanomaterials is affected by the route of exposure. However, few studies have addressed the exposure route or mechanism of toxicity of nanomaterials, particularly of nanowires, in living organisms. In this study, we therefore analyzed the main exposure route of AgNWs in vitro, using earthworms (Eisenia andrei) as a model system, via flow cytometry. We subsequently examined the in vivo toxicity of AgNWs to earthworms in soil. These tests revealed that intracellular esterase activity was correlated with adsorption of the nanowires to the surfaces of coelomocytes in vitro, and that in vivo cytotoxicity resulted mainly from oral, rather than dermal, exposure to the nanowires. Overall, shorter AgNWs (10 μm) were more toxic than longer AgNWs (20 μm). To our knowledge, this study is the first report regarding the ecotoxicity of nanowires to earthworms in soil. Our findings provide important information to help assess the risk of toxic AgNW contamination of soil ecosystems.