Plasma proteome of buffaloes

Area of Science:

• Veterinary proteomics
• Animal science
• Biochemistry

Background:

• Water buffaloes are economically significant for milk, meat, and leather production.
• Buffaloes are also vital donors of blood components.
• Proteomics is increasingly important in veterinary medicine for biopharmaceutical production and disease diagnosis.

Purpose of the Study:

• To identify and characterize proteins in Murrah buffalo (Bubalus bubalis) blood plasma.
• To utilize proteomic approaches for a deeper understanding of buffalo blood composition.
• To lay the groundwork for identifying molecular markers of zoonotic diseases in buffaloes.

Main Methods:

• Two-dimensional electrophoresis (2D electrophoresis) for protein separation.
• In-gel and in-solution protein digestion followed by mass spectrometry.
• Shotgun analysis and albumin depletion techniques were employed.

Main Results:

• Identification of 112 distinct protein spots and 35 individual proteins.
• Key identified proteins include albumin, fibrinogens, immunoglobulins, and serotransferrin.
• Many identified proteins are involved in complement system and coagulation cascade pathways.

Conclusions:

• This proteomic analysis provides a comprehensive profile of Murrah buffalo blood plasma proteins.
• The findings contribute to a better understanding of buffalo health and physiology.
• The study supports the search for novel molecular markers for zoonotic diseases in buffalo populations.