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Updated: Mar 3, 2026

Parallel High Throughput Single Molecule Kinetic Assay for Site-Specific DNA Cleavage
Published on: May 6, 2020
Site accessibility tailors DNA cleavage by restriction enzymes in DNA confined monolayers
Chiara Rotella1, Giovanni Doni, Alessandro Bosco
1Elettra Sincrotrone Trieste, s.s. 14 km 163.5 in Area Science Park, Trieste, Italy. pietro.parisse@elettra.eu.
Abstract:
Density-tunable nanografted monolayers (NAMs) of short oligonucleotide sequences on gold surfaces show novel properties that make them suitable for advanced biosensing applications, and in particular to study the effects of crowding and confinement on biomolecular interactions. Here, combining atomic force microscopy nanolithography, topography measurements and coarse-grained molecular dynamics simulations, we investigated restriction enzyme reaction mechanisms within confined DNA brushes highlighting the role played by the DNA sequence conformation and restriction site position along the chain, respectively, in determining the accessibility of the enzyme, and its consequent cleavage efficiency.
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