Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Codon usage in Pseudomonas aeruginosa.

S E West¹, B H Iglewski

¹Department of Microbiology and Immunology, School of Medicine and Dentistry, University of Rochester, NY 14642.

Nucleic Acids Research

|October 11, 1988

Summary

Pseudomonas aeruginosa exhibits highly biased codon usage, favoring codons with strong interactions. This bias, unlike in E. coli, reflects the genome

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Nutrient database improvement project: the influence of USDA quality and yield grade on the separable components and proximate composition of raw and cooked retail cuts from the beef chuck.

Meat science·2014

Same author

Innovative retail merchandising strategies to accommodate for the growing trend of heavier carcass weights in the United States.

Meat science·2011

Same author

Pseudomonas aeruginosa serological analysis in young children with cystic fibrosis diagnosed through newborn screening.

Pediatric pulmonology·2009

Same author

Suppression of cytotoxicity of diphtheria toxin by monoclonal antibodies against phosphatidylinositol phosphate.

Biophysical journal·2009

Same author

A DNA delivery system targeting dendritic cells for use in immunization against malaria: a rodent model.

Tanzania health research bulletin·2006

Same author

Early emergence of PNH-like T cells after allogeneic stem cell transplants utilising CAMPATH-1H for T cell depletion.

Bone marrow transplantation·2005

Area of Science:

Microbiology
Genomics
Molecular Biology

Background:

Codon usage bias is a significant factor in microbial gene expression.
Understanding codon bias in Pseudomonas aeruginosa is crucial for genetic engineering and synthetic biology applications.

Purpose of the Study:

To generate a comprehensive codon usage table for Pseudomonas aeruginosa.
To analyze the factors influencing codon usage bias in this bacterium.

Main Methods:

Analysis of available Pseudomonas aeruginosa genomic sequences.
Generation of a codon usage table based on gene sequences.
Comparison of codon usage patterns with related organisms like E. coli and yeast.

Main Results:

Pseudomonas aeruginosa displays extreme codon usage bias.
A preference for codons with stronger predicted codon-anticodon interactions was observed.
No direct correlation was found between codon usage patterns and predicted mRNA expressivity.
The high GC content (67.2%) of the P. aeruginosa genome influences codon choice, with cytosine being preferred at the third codon position.

Conclusions:

The codon usage bias in Pseudomonas aeruginosa is primarily driven by selection for optimal codon-anticodon interactions and the genome's high GC content.
This bias is distinct from that observed in E. coli and yeast.
Further research is needed to fully elucidate the relationship between codon usage and gene expression levels in P. aeruginosa.

Related Experiment Videos