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Updated: Mar 3, 2026

Waste Water Derived Electroactive Microbial Biofilms: Growth, Maintenance, and Basic Characterization
Published on: December 29, 2013
Bacterial community shift and improved performance induced by in situ preparing dual graphene modified bioelectrode
Junfeng Chen1, Yongyou Hu1, Lihua Zhang1
1The Key Lab of Pollution Control and Ecosystem Restoration in Industry Clusters, Ministry of Education, School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Centre, Guangzhou 510006, China.
Abstract:
Dual graphene modified bioelectrode (D-GM-BE) was prepared by in situ microbial-induced reduction of graphene oxide (GO) and polarity reversion in microbial fuel cell (MFC). Next Generation Sequencing technology was used to elucidate bacterial community shift in response to improved performance in D-GM-BE MFC. The results indicated an increase in the relative ratio of Proteobacteria, but a decrease of Firmicutes was observed in graphene modified bioanode (GM-BA); increase of Proteobacteria and Firmicutes were observed in graphene modified biocathode (GM-BC). Genus analysis demonstrated that GM-BE was beneficial to enrich electrogens. Typical exoelectrogens were accounted for 13.02% and 8.83% in GM-BA and GM-BC. Morphology showed that both GM-BA and GM-BC formed 3D-like graphene/biofilm architectures and revealed that the biofilm viability and thickness would decrease to some extent when GM-BE was formed. D-GM-BE MFC obtained the maximum power density by 124.58±6.32mWm-2, which was 2.34 times over C-BE MFC.

