Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Scanning Electron Microscopy01:07

Scanning Electron Microscopy

5.7K
A scanning electron microscope (SEM) is used to study the surface features of a sample by using an electron beam that scans the sample surface in a two-dimensional manner. Typically, areas between ~1 centimeter to 5 micrometers in width can be imaged. SEM can be used to image bacteria, viruses, tissues as well as larger samples like insects. Conventional SEM gives a magnification ranging from 20X to 30,000X and spatial resolution of 50 to 100 nanometers.
Fundamental Principles
Accelerated...
5.7K
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

17.4K
The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
17.4K
Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation01:26

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

875
Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy  (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....
875
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

7.5K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
7.5K
Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

3.0K
Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
3.0K
Atomic Force Microscopy01:08

Atomic Force Microscopy

4.6K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Photophysics and photochemistry of a prospective light-activated anticancer dirhodium complex.

Physical chemistry chemical physics : PCCP·2025
Same author

Observation of Light Localization at the Edges of Quasicrystal Waveguide Arrays.

Physical review letters·2025
Same author

Observation of Linear and Nonlinear Light Localization at the Edges of Moiré Arrays.

Physical review letters·2023
Same author

Observation of Edge Solitons in Topological Trimer Arrays.

Physical review letters·2022
Same author

The use of a piezoelectric force sensor in the magnetic force microscopy of thin permalloy films.

Ultramicroscopy·2020
Same author

Ion transmission through a dielectric hollow tip for scanning probe microscopy.

Micron (Oxford, England : 1993)·2018

Related Experiment Video

Updated: Mar 3, 2026

Scanning-probe Single-electron Capacitance Spectroscopy
10:53

Scanning-probe Single-electron Capacitance Spectroscopy

Published on: July 30, 2013

13.5K

Vacuum scanning capillary photoemission microscopy.

S A Aseyev1, A P Cherkun1, B N Mironov1

  • 1Institute of Spectroscopy, Russian Academy of Sciences, Troitsk, Moscow, 108840 Russia.

Ultramicroscopy
|April 29, 2017
PubMed
Summary
This summary is machine-generated.

We developed a conical capillary probe for scanning probe microscopy, enabling surface analysis via photoemission. This technique achieves submicron resolution for microstructure measurements and opens doors for advanced molecular ion studies.

Keywords:
Femtosecond laser radiationHollow tipPhotoemission microscopy

More Related Videos

Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser
09:00

Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser

Published on: June 28, 2018

10.5K
Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−
06:53

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Published on: July 27, 2018

9.2K

Related Experiment Videos

Last Updated: Mar 3, 2026

Scanning-probe Single-electron Capacitance Spectroscopy
10:53

Scanning-probe Single-electron Capacitance Spectroscopy

Published on: July 30, 2013

13.5K
Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser
09:00

Experimental Methods for Spin- and Angle-Resolved Photoemission Spectroscopy Combined with Polarization-Variable Laser

Published on: June 28, 2018

10.5K
Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−
06:53

Photoelectron Imaging of Anions Illustrated by 310 Nm Detachment of F−

Published on: July 27, 2018

9.2K

Area of Science:

  • Materials Science
  • Surface Science
  • Nanotechnology

Background:

  • Scanning probe microscopy (SPM) is crucial for surface analysis.
  • Characterizing microstructures requires high-resolution techniques.
  • Photoemission provides insights into material electronic properties.

Purpose of the Study:

  • To demonstrate a novel conical capillary probe for SPM.
  • To utilize photoemission for surface analysis with SPM.
  • To achieve submicron resolution for microstructure characterization.

Main Methods:

  • Employing a conical quartz capillary probe (2-µm aperture) in SPM.
  • Operating the probe in both shear force and photoelectron regimes.
  • Illuminating a gold-coated compact disc model substrate with a 400nm femtosecond laser.

Main Results:

  • Successfully measured the period of gold microstructure (1.6µm) using the conical probe in shear force mode.
  • Obtained surface topology images reflecting sample topography in shear force regime.
  • Recorded periodic photoelectron distributions resembling surface profiles in photoelectron regime, achieving submicron resolution.

Conclusions:

  • The conical capillary probe is effective for surface analysis in SPM.
  • The technique enables high-resolution imaging and microstructure measurement.
  • This method facilitates future studies on pulsed photodesorption of molecular ions with high spatial and element resolution.