Related Experiment Video

Updated: Mar 3, 2026

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer
06:01

A Genetically Engineered Mouse Model of Sporadic Colorectal Cancer

Published on: July 6, 2017

10.0K

Smad3 mutant mice develop colon cancer with overexpression of COX-2

Yu-Ping Zhu1, Zhuo Liu1, Zhi-Xuan Fu1

  • 1Department of Colorectal Cancer Surgery, Zhejiang Cancer Hospital, Hangzhou, Zhejiang 310022, P.R. China.

Oncology Letters
|April 30, 2017
PubMed

Insights

This study investigated cyclooxygenase-2 (COX-2) in Smad3 mutant mice, which develop colon cancer. Results show a link between Smad3 mutation, colon cancer development, and COX-2 overexpression.

Area of Science:

  • Oncology
  • Genetics
  • Molecular Biology

Background:

  • Colon cancer is a leading cause of cancer mortality.
  • Smad3 mutant mice are a model for colon cancer development.
  • Cyclooxygenase-2 (COX-2) is implicated in various cancers.

Purpose of the Study:

  • To elucidate the role of COX-2 in Smad3 mutant mice.
  • To investigate the correlation between Smad3 mutation, colon cancer, and COX-2 expression.
  • To identify the expression patterns of COX-2 in this colon cancer model.

Main Methods:

  • Generation of homozygous Smad3 (-/-) mutant mice.
  • Immunohistochemistry using COX-2 antibody.
  • Validation of results with reverse transcription-polymerase chain reaction (RT-PCR).

Main Results:

  • Successfully generated homozygous Smad3 mutant mice.
  • Identified an overexpression pattern of COX-2 in these mice.
  • Demonstrated a significant link between Smad3 mutation, colon cancer, and COX-2.

Conclusions:

  • Smad3 mutation is associated with colon cancer development in mice.
  • Overexpression of COX-2 is a key feature in Smad3 mutant mice with colon cancer.
  • COX-2 plays a role in the pathogenesis of colon cancer in this genetic model.
Keywords:
Smad3colon cancercyclooxygenase-2p53

More Related Videos

In vitro Organoid Culture of Primary Mouse Colon Tumors07:33

In vitro Organoid Culture of Primary Mouse Colon Tumors

Published on: May 17, 2013

36.0K
Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer06:19

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

Published on: July 18, 2017

10.8K

Related Concept Videos

mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.0K
Mouse Models of Cancer Study02:43

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...
6.6K