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Related Concept Videos

Key Techniques in Microbiology01:19

Key Techniques in Microbiology

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Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
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Methods of Sterilization II: Chemical Methods01:30

Methods of Sterilization II: Chemical Methods

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In healthcare, the chemical method of sterilization uses chemical sterilants to treat surgical instruments and medical supplies to help prevent the transmission of infectious pathogens to patients. Due to heat sensitivity, most medical supplies and equipment should not be exposed to high temperatures. These parts include rubber, plastic, glass, and other similar elements.
Using chemical sterilization rather than heat to clean out equipment is recommended. It eradicates and removes all bacteria,...
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Methods of Sterilization I: Physical Methods01:29

Methods of Sterilization I: Physical Methods

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As used in a healthcare facility, sterilization destroys all microorganisms through physical or chemical methods. The physical method includes steam, dry heat, boiling water, and radiation.
Steam sterilization uses non-toxic, low-cost moist heat in the form of saturated steam under pressure, which is fast, microbicidal, and sporicidal, and quickly warms and penetrates fabrics. Autoclaves, or steam sterilizers, expose each item to direct steam contact for a predetermined time at the necessary...
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Asepsis01:28

Asepsis

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The condition of being free from disease-causing living pathogens is asepsis. Aseptic techniques include a set of standard practices to achieve asepsis. An example is the regular environmental cleaning of all parts of the healthcare facility and hand hygiene at home before preparing or eating food. Medical and surgical asepsis in healthcare practice protects patients from harmful pathogens, minimizes the risk of contamination of susceptible sites, and reduces the risk of infection transmission.
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Cleaning, Sterilization, and Disinfection01:30

Cleaning, Sterilization, and Disinfection

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Cleaning, disinfection, and sterilization are the methods that help to break the infection chain and prevent disease.
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
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Techniques for Isolation of Pure Cultures01:24

Techniques for Isolation of Pure Cultures

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Microorganisms are routinely cultured in the laboratory using various techniques to isolate, grow, and quantify them for further study. These methods rely on inoculating microorganisms into a suitable growth medium under aseptic conditions to prevent contamination. Depending on the objective, inoculation can involve direct transfer or the use of diluted bacterial suspensions as the inoculum.Streak-Plate Method for IsolationThe streak-plate method is a common technique for obtaining pure...
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Aseptic Laboratory Techniques: Volume Transfers with Serological Pipettes and Micropipettors
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Back to Basics: Sterile Technique.

Lisa Spruce

    AORN Journal
    |April 30, 2017
    PubMed
    Summary
    This summary is machine-generated.

    Sterile technique is crucial for patient safety during surgery, preventing microbial transmission. All perioperative team members must master aseptic principles to maintain a sterile environment and ensure patient well-being.

    Keywords:
    glovingsterile drapessterile fieldsterile techniquesurgical site infection

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    Area of Science:

    • Surgical Patient Safety
    • Infection Prevention and Control

    Background:

    • Sterile technique is a fundamental patient safety principle in surgical settings.
    • It is critical for minimizing the risk of microbial transmission to patients during operative procedures.
    • Maintaining a sterile environment is a key skill for all perioperative personnel.

    Purpose of the Study:

    • To review the core principles of sterile technique.
    • To emphasize the importance of adherence to aseptic principles by all perioperative team members.
    • To provide foundational knowledge for individuals entering the perioperative environment.

    Main Methods:

    • Review of established sterile technique protocols and guidelines.
    • Discussion of the significance of aseptic practices in surgical patient care.
    • Educational content aimed at perioperative team members.

    Main Results:

    • Understanding sterile technique principles is vital for healthcare professionals.
    • Adherence to aseptic principles by the entire perioperative team is essential.
    • Mastery of sterile technique directly impacts patient safety outcomes.

    Conclusions:

    • Sterile technique is indispensable for reducing surgical site infections.
    • Continuous education and reinforcement of aseptic principles are necessary for all perioperative staff.
    • Upholding sterile technique standards is a shared responsibility crucial for patient safety.