Combined recovery of biological and fibre traces

We present a method in which DNA and fibre traces are jointly recovered by taping. The DNA traces are isolated by standard laboratory procedures. Fibre traces are isolated afterwards in order to improve efficiency. Two tests have been carried out to evaluate the suitability of the presented method. In the first test, possible changes in appearance of fibres due to the DNA isolation procedures are investigated. In the second test, the recovery of fibres from a contaminated surface and their possible loss due to the DNA isolation procedure are investigated. It is concluded that polyester fibres are hardly affected by the DNA isolation procedure. In contrast, a relatively large number of the investigated cotton fibres were altered. The observed differences do not indicate a structural damage to the fibre or the dyes, but rather the washing-out of some components. The observed changes may require that fibres from a known source are also exposed to the DNA isolation procedures to assess the induced changes, but do not prevent a meaningful comparison. The recovery of fibres is slightly lower than the routine procedures for fibre recovery. Therefore, it was decided to perform extra taping of the recipient in cases where fibre investigation is requested. During DNA-isolation, some of the fibres present are released from the tapes. These fibres are not lost however, as they can be found on the filter in the used DNA isolation vials.