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Oxidative stress in Mayaro virus infection
Fernanda Caetano Camini1, Camila Carla da Silva Caetano1, Letícia Trindade Almeida1
1Núcleo de Pesquisas em Ciências Biológicas, NUPEB, Universidade Federal de Ouro Preto, Ouro Preto, Minas Gerais, Brazil.
Virus Research
|April 30, 2017
Summary
Mayaro virus (MAYV) infection causes oxidative stress in host cells, marked by increased reactive oxygen species (ROS) and damage markers, despite boosted antioxidant defenses. This study reveals key mechanisms in MAYV pathogenesis.
Area of Science:
- Virology
- Pathogenesis
- Oxidative Stress Biology
Background:
- Mayaro virus (MAYV) is a neglected tropical arbovirus causing febrile illness and arthritis.
- Viral pathogenesis is often linked to oxidative stress, involving reactive oxygen species (ROS) and altered antioxidant defenses.
Purpose of the Study:
- To investigate MAYV-induced oxidative stress in host cells.
- To characterize the impact of MAYV infection on ROS production, oxidative stress markers, and antioxidant capacity.
Main Methods:
- Monitoring ROS production, malondialdehyde (MDA), protein carbonyls, and glutathione redox state (GSH/GSSG) in MAYV-infected HepG2 cells.
- Assessing superoxide dismutase (SOD) and catalase (CAT) activities and total glutathione levels.
- Evaluating ROS, MDA, and SOD activity in MAYV-infected J774 cells.
Main Results:
- MAYV infection significantly increased ROS, MDA, and protein carbonyl levels in HepG2 cells.
- A decreased GSH/GSSG ratio indicated oxidative stress, despite increased SOD, CAT activities, and total glutathione.
- Similar oxidative stress patterns (increased ROS, MDA, SOD) were observed in MAYV-infected J774 cells.
Conclusions:
- MAYV infection induces significant oxidative stress in host cells, characterized by ROS overproduction.
- Increased antioxidant defenses do not fully counteract the oxidative damage caused by MAYV.
- This study is the first to report the involvement of oxidative stress in MAYV infection, offering insights into viral pathogenesis.
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