Temporal and spatiotemporal variability in comprehensive forearm skin microcirculation assessment during occlusion protocols

Forearm skin hyperemia during release after brachial occlusion has been proposed for evaluating peripheral arterial disease and endothelial dysfunction. We used a novel fiberoptic system integrating Laser Doppler Flowmetry and Diffuse Reflectance Spectroscopy for a comprehensive pointwise model based microcirculation characterization. The aim was to evaluate and compare the temporal and the spatiotemporal variabilities in forearm skin microcirculation parameters (speed resolved perfusion; low speed <1mm/s, Perf_{SR, <1}; mid-speed 1-10mm/s, high speed >10mm/s, and total perfusion (Perf_{SR, tot}); the concentration and oxygenation of red blood cells, C_RBC and S_O2). Ten healthy subjects underwent arterial and venous forearm occlusions (AO, VO), repeated within one week. The repeatability was calculated as the coefficient of variation (CV) and the agreement as the intra-class correlation coefficient (ICC). The temporal CVs for conventional perfusion, Perf_conv, Perf_{SR, tot}, C_RBC and S_O2 were 14%, 12%, 9% and 9%, respectively, while the ICC were >0.75 (excellent). The perfusion measures generally had a higher spatiotemporal than temporal variability, which was not the case for S_O2 and C_RBC. The corresponding spatiotemporal CVs were 33%, 32%, 18% and 15%, respectively. During VO, C_RBC had a CV<35% and ICC>0.40 (fair-good), and after release this was the case for C_RBC (AO and VO), S_O2 (VO) and Perf_{SR, <1} (VO). In conclusion, the skin microcirculation parameters showed excellent temporal repeatability, while the spatiotemporal repeatability especially for perfusion was poorer. The parameters with acceptable repeatability and fair-good agreement were: C_RBC during and after release of VO, the Perf_{SR, <1} after release of VO, the S_O2 and the C_RBC after release of AO. However, the value of these parameters in discriminating endothelial function remains to be studied.