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RCCD1 depletion attenuates TGF-β-induced EMT and cell migration by stabilizing cytoskeletal microtubules in NSCLC

Junyu Wu1, Zhimin He2, Xiao-Mei Yang3

  • 1Research Centre for Translational Medicine at East Hospital, School of Life Sciences and Technology, Tongji University, Shanghai 200092/200120, China; Department of Basic Medical Sciences, School of Medicine, Tsinghua University, Beijing 100084, China.

Cancer Letters
|April 30, 2017
PubMed

Insights

RCCD1 protein promotes lung cancer cell migration by regulating microtubule stability and epithelial-to-mesenchymal transition (EMT). Inhibiting RCCD1 may offer a new strategy for treating non-small cell lung cancer (NSCLC).

Area of Science:

  • Oncology
  • Cell Biology
  • Molecular Medicine

Background:

  • Lung cancer exhibits high metastatic potential, driven by cell motility regulated by factors like TGF-β.
  • Epithelial-to-mesenchymal transition (EMT) and microtubule stability are crucial for cancer cell migration.
  • The role of RCCD1 in non-small cell lung cancer (NSCLC) metastasis requires further elucidation.

Purpose of the Study:

  • To investigate the role of RCCD1 in NSCLC progression and metastasis.
  • To elucidate the molecular mechanisms by which RCCD1 influences cancer cell motility and EMT.
  • To assess the therapeutic potential of targeting RCCD1 in NSCLC.

Main Methods:

  • Analysis of RCCD1 expression in NSCLC patient tissues and correlation with prognosis.
  • siRNA-mediated depletion of RCCD1 to assess its impact on lung cancer cell migration.
  • Investigation of RCCD1's interaction with JMJD5 and its effect on microtubule acetylation and stability.
  • Assessment of TGF-β-induced EMT markers and cell migration following RCCD1 depletion.

Main Results:

  • RCCD1 is highly expressed in NSCLC tumors and associated with poor prognosis.
  • RCCD1 depletion significantly inhibits lung cancer cell migration.
  • RCCD1 modulates microtubule stability via interaction with JMJD5, leading to increased acetylated α-tubulin.
  • RCCD1 depletion attenuates TGF-β-induced EMT and cell migration.

Conclusions:

  • RCCD1 acts as a novel regulator of TGF-β-induced EMT in NSCLC.
  • RCCD1 promotes lung cancer cell migration by stabilizing microtubules.
  • Targeting RCCD1 presents a potential therapeutic strategy for NSCLC.
Keywords:
Lung cancerMicrotubule stabilityRCCD1TGF-βα-tubulin acetylation

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