Related Experiment Video

Updated: Mar 3, 2026

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
07:23

Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay

Published on: June 9, 2011

19.1K

Enterovirus D68 Subclade B3 Strain Circulating and Causing an Outbreak in the United States in 2016

Guiqing Wang1,2, Jian Zhuge3, Weihua Huang4

  • 1Department of Pathology, New York Medical College, Valhalla, New York, USA. guiqing_wang@nymc.edu.

Scientific Reports
|April 30, 2017
PubMed

Insights

Enterovirus D68 (EV-D68) re-emerged in 2016 with a new B3 subclade strain causing an outbreak in New York. This highlights the genetic diversity and outbreak potential of EV-D68 strains, necessitating global surveillance.

Area of Science:

  • Virology
  • Epidemiology
  • Genomics

Background:

  • The United States experienced a nationwide Enterovirus D68 (EV-D68) outbreak in 2014.
  • Limited EV-D68 detection was reported in 2015 and 2016.
  • EV-D68 is a significant respiratory pathogen.

Purpose of the Study:

  • To analyze the prevalence and genetic characteristics of EV-D68 in the Lower Hudson Valley, New York, during 2015-2016.
  • To identify the circulating EV-D68 strain and its genetic relationship to previous strains.

Main Methods:

  • Analysis of 749 nasopharyngeal specimens collected in 2015-2016 using EV-D68-specific real-time reverse transcription polymerase chain reaction (rRT-PCR).
  • Whole-genome sequencing of detected EV-D68 strains using metagenomic next-generation sequencing.
  • Comparative genomic analysis to determine strain lineage and divergence.

Main Results:

  • No EV-D68 detected in 199 specimens from 2015.
  • EV-D68 detected in 0.9% of samples from January-May 2016 and 36.0% from July-October 2016.
  • A novel EV-D68 strain (subclade B3) was identified, causing 160 confirmed cases in a 2016 outbreak.
  • The new strain showed 3.2-4.8% nucleotide divergence from the 2014 B1 subclade strains.

Conclusions:

  • A new EV-D68 subclade B3 strain emerged and caused a significant outbreak in New York in 2016.
  • EV-D68 strains exhibit genetic variability and the potential for recurrent outbreaks.
  • Enhanced global surveillance is crucial for monitoring the circulation of diverse EV-D68 strains.

More Related Videos

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR12:32

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. Part III. Virus Detection by RT-qPCR

Published on: January 16, 2016

13.3K
EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples10:48

EPA Method 1615. Measurement of Enterovirus and Norovirus Occurrence in Water by Culture and RT-qPCR. I. Collection of Virus Samples

Published on: March 28, 2015

12.9K

Related Concept Videos

Steps in Outbreak Investigation01:18

Steps in Outbreak Investigation

In the ever-evolving field of public health, statistical analysis serves as a cornerstone for understanding and managing disease outbreaks. By leveraging various statistical tools, health professionals can predict potential outbreaks, analyze ongoing situations, and devise effective responses to mitigate impact. For that to happen, there are a few possible stages of the analysis:
653
Viral Recombination00:57

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.
25.4K
Vaccinations01:51

Vaccinations

Overview
52.8K