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The Plant Infection Test: Spray and Wound-Mediated Inoculation with the Plant Pathogen Magnaporthe Grisea
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ER retention receptor, MoERR1 is required for fungal development and pathogenicity in the rice blast fungus,

Jaeduk Goh1,2, Junhyun Jeon3, Yong-Hwan Lee4,5

  • 1Department of Agricultural Biotechnology, College of Agriculture and Life Sciences, Seoul National University, Seoul, 08826, Korea.

Scientific Reports
|April 30, 2017
PubMed

Insights

Magnaporthe oryzae ER retention receptor 1 (MoERR1) is crucial for endoplasmic reticulum (ER) function and fungal pathogenicity. Disruption of MoERR1 impairs ER integrity, leading to non-pathogenic fungal development.

Area of Science:

  • Molecular Biology
  • Mycology
  • Cell Biology

Background:

  • The endoplasmic reticulum (ER) is vital for cellular function and integrity.
  • ER retention receptors regulate ER homeostasis.
  • Magnaporthe oryzae (M. oryzae) is a significant fungal pathogen.

Purpose of the Study:

  • To investigate the role of ER retention receptors in M. oryzae.
  • To characterize the function of MoERR1, an ortholog of Saccharomyces cerevisiae ERD2.

Main Methods:

  • Insertional mutagenesis in M. oryzae.
  • Gene disruption and phenotypic analysis.
  • Green Fluorescent Protein (GFP) tagging and localization studies.

Main Results:

  • MoERR1, an ER retention receptor, was identified as a pathogenicity gene.
  • MoERR1 disruption caused growth retardation, reduced conidia production, and aberrant morphology.
  • MoERR1 mutant appressoria failed to penetrate plant tissues, rendering the fungus non-pathogenic.
  • MoERR1 deficiency led to abnormal ER structure and misregulated unfolded protein response.

Conclusions:

  • MoERR1 is essential for ER function and integrity in M. oryzae.
  • MoERR1-mediated ER functionalities are critical for fungal development and pathogenesis.

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