Related Experiment Video
Updated: Mar 3, 2026

Transcriptome Profiling of In-Vivo Produced Bovine Pre-implantation Embryos Using Two-color Microarray Platform
Published on: January 30, 2017
Potential Involvement of SCF-Complex in Zygotic Genome Activation During Early Bovine Embryo Development
Veronika Benesova1,2, Veronika Kinterova3,4, Jiri Kanka3
1Laboratory of Developmental Biology, Institute of Animal Physiology and Genetics, Academy of Science of Czech Republic, v.v.i., Libechov, Czech Republic. benesova@iapg.cas.cz.
Abstract:
Proper timing of degradation of maternal protein reserves is important for early embryonic development. The major modification that triggers proteins to degradation is ubiquitination, mediated by ubiquitin-proteolytic system. We focus here on Skp 1-Cul 1-F-box complex (SCF-complex), E3 ubiquitin-ligase, a part of ubiquitin-proteolytic system, which transfer ubiquitin to the substrate protein. We describe in this chapter the methods for the characterization of the expression profile of mRNA and protein of invariant members of SCF-complex and for the definition of SCF-complex activity.
More Related Videos
Related Concept Videos
Zygotic Development And Stem Cell Formation
Cleavage and Blastulation
Gene Regulation During Sporulation
Fertilization
Gastrulation

