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Custom Engineered Tissue Culture Molds from Laser-etched Masters
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Laser-Etched Designs for Molding Hydrogel-Based Engineered Tissues.

Fabiola Munarin1, Nicholas J Kaiser1, Tae Yun Kim2

  • 11 School of Engineering, Brown University , Providence, Rhode Island.

Tissue Engineering. Part C, Methods
|May 2, 2017
PubMed
Summary

This study presents a low-cost, accessible method for fabricating custom elastomeric molds for engineered tissues. These molds enable precise control over tissue structure, improving cardiac tissue function and paving the way for regenerative medicine applications.

Keywords:
PDMS moldscardiac regenerationhydrogel polymersstem cellstissue engineering

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Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Tissue Engineering

Background:

  • Commercially available molds for engineered tissues have limited adaptability for specific applications.
  • Customizable scaffolds are crucial for regenerative medicine and in vitro tissue culture.
  • Elastomeric molds offer potential for tailored tissue geometries.

Purpose of the Study:

  • To develop a rapid, low-cost method for fabricating customizable elastomeric molds for tissue engineering.
  • To demonstrate the utility of these molds in creating engineered cardiac tissues with controlled morphology and function.
  • To present techniques for in vivo implantation of engineered cardiac tissues.

Main Methods:

  • Fabrication of poly(dimethylsiloxane) (PDMS) molds using a replica molding technique from laser-etched acrylic masters.
  • Utilizing PDMS molds for the production and culture of engineered cardiac tissues from human-induced pluripotent stem cell-derived cardiomyocytes.
  • Developing and presenting in vivo implantation techniques for engineered cardiac tissues in a rat myocardial infarction model.

Main Results:

  • Achieved ∼0.2 mm resolution in PDMS mold fabrication via laser etching.
  • Demonstrated tight control over engineered cardiac tissue morphology and anisotropy, modulating cell alignment and conduction properties.
  • Successfully modulated reentrant arrhythmias in engineered cardiac tissues through controlled morphology.
  • Developed and presented techniques for in vivo handling and implantation of engineered cardiac tissues.

Conclusions:

  • The presented mold fabrication method is straightforward, low-cost, and accessible, enabling rapid design iteration for tissue engineering applications.
  • Customizable PDMS molds facilitate precise control over engineered tissue properties, impacting cell behavior and tissue function.
  • The developed techniques support the advancement of regenerative medicine and surgical applications for engineered tissues.