Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Chloroquine interaction with inflammatory human polymorphonuclear leucocytes.

M Raghoebar1, J A Huisman, W B van den Berg

  • 1Department of Pharmacology, University of Nijmegen, The Netherlands.

Agents and Actions
|July 1, 1988
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A Three-Dimensional View on Soil Biogeochemistry: A Dataset for a Forested Headwater Catchment.

Journal of environmental quality·2017
Same author

Comparison of three-factor and four-factor prothrombin complex concentrates regarding reversal of the anticoagulant effects of rivaroxaban in healthy volunteers.

Journal of thrombosis and haemostasis : JTH·2014
Same author

Performance of four current risk algorithms in predicting cardiovascular events in patients with early rheumatoid arthritis.

Annals of the rheumatic diseases·2014
Same author

Markers of inflammation are negatively correlated with serum leptin in rheumatoid arthritis.

Annals of the rheumatic diseases·2005
Same author

A simplified disease activity index for rheumatoid arthritis for use in clinical practice.

Rheumatology (Oxford, England)·2003
Same author

Modified sharp method: factors influencing reproducibility and variability.

Seminars in arthritis and rheumatism·2001

Inflammation reduces intracellular chloroquine (CQ) concentration in polymorphonuclear leucocytes (PMNs) by 30-40%. Mechanisms include PMN volume expansion, pH changes, and degranulation, impacting CQ distribution in disease states.

Area of Science:

  • Pharmacology
  • Immunology
  • Cell Biology

Background:

  • Chloroquine (CQ) is an antimalarial drug with potential anti-inflammatory properties.
  • Polymorphonuclear leucocytes (PMNs) are key immune cells involved in inflammatory responses.
  • Understanding CQ's interaction with PMNs is crucial for its therapeutic applications in inflammatory diseases.

Purpose of the Study:

  • To quantify the in vitro association of radiolabelled CQ with normal and inflammatory PMNs.
  • To investigate the mechanisms underlying altered CQ intracellular concentrations in inflamed PMNs.
  • To explore the implications of these findings for rheumatic diseases.

Main Methods:

  • Development of a ligand-association assay to measure cell association and intracellular CQ concentration.

Related Experiment Videos

  • In vitro incubation of PMNs with radiolabelled CQ under normal and inflammatory conditions.
  • Association experiments with transport inhibitors to elucidate uptake mechanisms.

    • Main Results:

    • Inflammatory stimuli altered CQ interaction with PMNs, reducing intracellular CQ concentration by 30-40%.
    • Both diffusive uptake and carrier-mediated transport contribute to CQ accumulation in inflammatory PMNs.
    • PMN volume expansion, altered cytoplasmic pH, Na+/H+ antiport activation, and degranulation were identified as potential mechanisms.

    Conclusions:

    • Inflammatory conditions significantly decrease intracellular CQ concentration in PMNs.
    • Multiple mechanisms, including changes in cell volume, pH, ion transport, and degranulation, contribute to this reduction.
    • These findings provide insights into CQ's pharmacokinetics in inflammatory diseases, particularly rheumatic conditions.