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Experimental design data for the biosynthesis of citric acid using Central Composite Design method
Anand Kishore Kola1, Mallaiah Mekala1, Venkat Reddy Goli1
1Department of Chemical Engineering, National Institute of Technology, Warangal, Telangana 506004, India.
Data in Brief
|May 2, 2017
Summary
This study optimized citric acid production using *A. niger* fungi. Statistical methods identified optimal conditions, yielding 82.0 g/L of citric acid, demonstrating effective microbial fermentation.
Area of Science:
- Biotechnology
- Industrial Microbiology
- Biochemical Engineering
Background:
- Citric acid is a key industrial chemical produced via microbial fermentation.
- Optimizing fermentation parameters is crucial for maximizing yield and efficiency.
- Aspergillus niger is a widely used filamentous fungus for citric acid production.
Purpose of the Study:
- To statistically design and optimize variables for citric acid production from sucrose using *A. niger* NCIM 705.
- To develop a predictive model for citric acid fermentation based on key process parameters.
- To identify optimal conditions for maximizing citric acid yield.
Main Methods:
- Response Surface Methodology (RSM) with Central Composite Design (CCD) was employed.
- Six variables were investigated: sucrose concentration, initial pH, temperature, incubation time, stirrer speed, and oxygen flow rate.
- Statistical modeling was used to analyze experimental data and predict optimal conditions.
Main Results:
- A statistical model was developed to describe citric acid production.
- Optimal conditions determined: 163.6 g/L sucrose, pH 5.26, 247.78 rpm, 8.18 days, 30.06°C, and 1.35 lpm oxygen flow.
- Predicted maximum citric acid yield was 86.42 g/L, with experimental validation yielding 82.0 g/L.
Conclusions:
- The developed statistical model accurately represents the experimental data for citric acid production.
- The identified optimal conditions significantly enhance citric acid yield by *A. niger*.
- This study provides a robust framework for optimizing microbial citric acid fermentation.
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