Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Next-generation Sequencing

Next-generation Sequencing

The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....

Real Time RT-PCR

Real Time RT-PCR

Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Mechanism-Informed Rules Tunably Balance Novelty and Feasibility of Predicted Enzymatic Reactions.

Journal of chemical information and modeling·2026

Same author

Thousandfold Expansion Microscopy.

bioRxiv : the preprint server for biology·2026

Same author

Isotropic shrinkage of patterned vacancies enables three-dimensional nanoprecise metastructures for visible light applications.

Nature photonics·2026

Same author

Mechanism-informed rules tunably balance novelty and feasibility of predicted enzymatic reactions.

bioRxiv : the preprint server for biology·2026

Same author

Mutation E300 recommended by protein language models gives ChrimsonR amplified photocurrent response.

bioRxiv : the preprint server for biology·2026

Same author

Unified Transcriptome and Mechanics Map of the Intact Mammalian Preimplantation Embryo In Situ.

bioRxiv : the preprint server for biology·2026

Same journal

Systematic design of auxotrophic strains and media conditions to probe metabolic functions in E. coli.

PLoS computational biology·2026

Same journal

Neuronal excitability and parameter variability in the Hodgkin-Huxley model.

PLoS computational biology·2026

Same journal

Delayed reward information is underweighted in reinforcement learning with dispersed feedback.

PLoS computational biology·2026

Same journal

GHF-ACL: A novel contrastive learning framework with multi-order graph structures for herb-disease association prediction.

PLoS computational biology·2026

Same journal

GATE: Adaptive learning with working memory by information gating in multi-lamellar hippocampal formation.

PLoS computational biology·2026

Same journal

Evaluating vectors for the design of a spillover-disrupting Lassa virus transmissible vaccine.

PLoS computational biology·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Mar 3, 2026

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

Nucleotide-time alignment for molecular recorders.

Thaddeus R Cybulski¹, Edward S Boyden^2,3,4, George M Church^5,6,7

¹Department of Physical Medicine and Rehabilitation, Rehabilitation Institute of Chicago, Northwestern University, Chicago, Illinois, United States of America.

Plos Computational Biology

|May 2, 2017

Summary

This summary is machine-generated.

This study introduces a novel algorithm for DNA-based neural recording, enabling precise estimation of neural activity over time. The method enhances DNA polymerase recording accuracy and guides future molecular recorder development.

More Related Videos

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Published on: March 20, 2021

Related Experiment Videos

Last Updated: Mar 3, 2026

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Dual DNA Rulers to Study the Mechanism of Ribosome Translocation with Single-Nucleotide Resolution

Published on: July 8, 2019

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Simultaneous Mapping and Quantitation of Ribonucleotides in Human Mitochondrial DNA

Published on: November 14, 2017

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

DNA Tension Probes to Map the Transient Piconewton Receptor Forces by Immune Cells

Published on: March 20, 2021

Area of Science:

Neuroscience
Molecular Biology
Bioinformatics

Background:

Novel neural recording techniques are needed for massive-scale, single-cell resolution monitoring.
DNA polymerase can be used to record intracellular calcium levels, storing neural activity in DNA.
Estimating the timing of DNA incorporation is challenging with current methods.

Purpose of the Study:

To develop an algorithm for estimating timing data in DNA-based neural recordings.
To address challenges like determining recording start times and DNA polymerase pausing.
To optimize DNA polymerase characteristics for improved molecular recorder performance.

Main Methods:

A Dynamic Time Warping-based algorithm was developed to estimate nucleotide incorporation times.
The algorithm exploits correlations between neural activity and experimental variables.
The method was applied to in silico motor control experiments and analyzed polymerase properties.

Main Results:

The algorithm accurately estimates DNA-based record timings within 10% of the recording window.
It successfully estimates unobserved incorporation times and neural tuning, like motor cortical cell directionality.
Useful ranges for DNA polymerase kinetic and error properties were identified.

Conclusions:

The developed algorithm significantly improves the accuracy and efficiency of DNA-based neural recording.
This work provides crucial guidance for protein engineering in developing advanced molecular recorders.
The algorithm represents a significant advancement for molecular recording technology and dynamic alignment techniques.