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Genetic characterization of a Neisseria meningitidis cluster in Queensland, Australia

N R Abady1, C J D Guglielmino2, R M Graham2

  • 1a Microbial Gene Research and Resources Facility, School of Natural Sciences, Griffith University, Queensland, Australia.

Canadian Journal of Microbiology
|May 2, 2017
PubMed

Insights

Invasive meningococcal disease (IMD) in Australia is primarily caused by Neisseria meningitidis serogroups B and C. Genomic analysis of a 2002 cluster revealed a serogroup Y case was unrelated to the serogroup C cases.

Area of Science:

  • Microbiology
  • Epidemiology
  • Genomics

Background:

  • Neisseria meningitidis serogroups B and C are the leading causes of invasive meningococcal disease (IMD) in Australia.
  • Serogroup B strains show an increasing prevalence in recent years.
  • Serogroup Y typically causes sporadic IMD cases in Australia.

Purpose of the Study:

  • To investigate a cluster of invasive meningococcal disease cases in Queensland in 2002.
  • To determine the genomic relationship between serogroup C and serogroup Y isolates within the cluster.

Main Methods:

  • Genomic analysis including antigen finetyping, multilocus sequence typing (MLST), and core genome MLST.
  • Molecular detection for diagnosis.

Main Results:

  • A cluster of 4 IMD cases included 3 serogroup C and 1 serogroup Y infection.
  • Genomic analysis confirmed the serogroup Y case was not a result of a capsule switch from the serogroup C cases.
  • One serogroup C isolate exhibited a complete deletion of the porA gene sequence.

Conclusions:

  • The serogroup Y case in the Queensland cluster was epidemiologically linked but genomically distinct from the serogroup C cases.
  • The findings highlight the genetic diversity within Neisseria meningitidis outbreaks.
  • PorA gene deletions can occur in circulating serogroup C strains.
Keywords:
Neisseria meningitidismutant porAoutbreakporA mutantséquençage du génome entierwhole genome sequencingéclosion

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