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Virus inactivation in hemoglobin solutions by heat
T N Estep1, M K Bechtel, T J Miller
1Travenol Laboratories, Inc., Round Lake, Illinois 60073.
Biomaterials, Artificial Cells, and Artificial Organs
|January 1, 1988
Summary
Heat treatment of hemoglobin solutions at 60°C effectively inactivates viruses while preserving protein structure and function. This method offers a practical approach for viral inactivation in hemoglobin-based products.
Area of Science:
- Biochemistry
- Virology
- Biotechnology
Background:
- Hemoglobin solutions are explored as potential blood substitutes.
- Viral contamination is a significant safety concern for therapeutic protein products.
- Effective viral inactivation methods are crucial for product safety.
Purpose of the Study:
- To assess the feasibility of heat treatment for viral inactivation in hemoglobin solutions.
- To evaluate the impact of heat treatment on hemoglobin stability and function.
Main Methods:
- Incubation of deoxyhemoglobin solutions at 60°C for 10 hours at pH 7.5.
- Assessment of protein structure and function post-incubation.
- Testing viral inactivation using model viruses and human immunodeficiency virus (HIV).
Main Results:
- Deoxyhemoglobin structure and function were well-preserved under the tested conditions.
- Rapid inactivation of model viruses and HIV was observed.
- Heat treatment at 60°C demonstrated significant viral clearance.
Conclusions:
- Heat treatment at 60°C is a feasible and effective method for viral inactivation in hemoglobin solutions.
- This process maintains the integrity of deoxyhemoglobin.
- The findings support the use of thermal processing for ensuring the safety of hemoglobin-based products.