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Related Experiment Video

Updated: May 6, 2026

Visualization of Caenorhabditis elegans Cuticular Structures Using the Lipophilic Vital Dye DiI
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dpy-13: a nematode collagen gene that affects body shape.

N von Mende1, D M Bird, P S Albert

  • 1Division of Biological Sciences, University of Missouri, Columbia 65211.

Cell
|November 18, 1988
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Summary

The Caenorhabditis elegans dpy-13 gene, a collagen family member, influences body shape. Its characterization involved transposon tagging and DNA sequencing, revealing its role in nematode development.

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Area of Science:

  • Genetics
  • Molecular Biology
  • Developmental Biology

Background:

  • Mutations in the Caenorhabditis elegans dpy-13 gene cause a "dumpy" phenotype, characterized by a short, chunky body shape.
  • Understanding the molecular basis of dpy-13 is crucial for comprehending nematode morphology and development.

Purpose of the Study:

  • To clone and characterize the wild-type dpy-13 gene.
  • To determine the molecular nature of dpy-13 and its relationship to other genes.

Main Methods:

  • Tc1 transposon tagging was used to identify and isolate the dpy-13 gene.
  • Chromosomal walking was employed to clone the wild-type dpy-13 gene, located 11 kb from the ama-1 gene.
  • DNA sequencing was performed to analyze the gene's structure and identify mutations.

Main Results:

  • The dpy-13 gene was cloned and found to be approximately 1.2 kb in length.
  • Three transposon insertion sites were mapped near the 5' end of the transcribed region.
  • DNA sequencing revealed that dpy-13 belongs to the collagen multi-gene family, encoding a 302-amino acid polypeptide.

Conclusions:

  • The dpy-13 gene encodes a collagen, likely involved in structural integrity and body shape determination in C. elegans.
  • A unique 146 bp sequence within dpy-13 hybridizes to a 1 kb mRNA, indicating specific regulatory or functional roles.