Related Experiment Video
Updated: Mar 3, 2026

Digital Inline Holographic Microscopy DIHM of Weakly-scattering Subjects
Published on: February 8, 2014
Three-dimensional image reconstruction of macroscopic objects from a single digital hologram using stereo disparity
Abstract:
We present depth extraction of macroscopic three-dimensional (3D) objects from a single digital hologram using stereo disparity. The method does not require the phase information of the hologram but two perspectives of the scene, which are easily obtained by dividing the hologram into two parts (two apertures) before the reconstruction. Variation of the hologram division is countless since each piece of a single hologram contains all the information regarding the scene; therefore, stereo disparity can be calculated along any arbitrary direction. We investigated the effects of gradual and sharp divisions of the holograms for the disparity map calculations, specifically for divisions in the vertical, horizontal, and diagonal directions. After obtaining the depth map from the stereo images, a regular two-dimensional image of the object is merged with the depth information to form 3D visualization of the object. Holograms were recorded with a rigid endoscope, and experimentally obtained depth profiles of the objects are in very good agreement with the actual profiles.
Related Concept Videos
Three-Dimensional Microscopy in Microbiology
Electron Microscope Tomography and Single-particle Reconstruction
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Super-resolution Fluorescence Microscopy

