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An enzyme which specifically splits a covalent bond between picornaviral RNA and VPg
Drygin YuF1, A A Shabanov, A A Bogdanov
1A.N. Belozersky Laboratory of Molecular Biology and Bioorganic Chemistry, Moscow State University, USSR.
FEBS Letters
|November 7, 1988
Abstract:
An enzyme which specifically splits a covalent bond between VPg and picornaviral RNAs (tentatively designated Y-pUpN PDE) has been partially purified from mouse ascites Krebs II cells. Using substrates labelled in vitro with 125I-Bolton-Hunter reagent and a new assay based on Kieselgel thin-layer chromatography, several biochemical characteristics of the enzyme have been determined, depending on the pH and on Mg2+, K+, spermidine and PEG concentrations, etc. We found that the enzyme does not 'unlink' VPg from comoviral RNA. We suggest that Y-pUpN PDE represents a new class of enzymes.