Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Health risk profiles of heavy metals and radionuclides in geothermal waters and groundwater, Kanchanaburi Province, Thailand: An interannual comparative baseline assessment.

The Science of the total environment·2026
Same author

Long-Term Stable Wearable Electrochemical Aptamer Sensor for Cortisol in Artificial Sweat Based on a Gold Nanoparticle-Carboxymethyl Cellulose-Methylene Blue Interface.

ACS omega·2026
Same author

A disposable molecularly imprinted electrochemical sensing electrode for sub-picomolar histamine detection based on a Prussian blue-embedded imprinted polypyrrole coating on AuNPs@Ti<sub>3</sub>C<sub>2</sub>T<sub>x</sub> MXene nanocomposite.

Food chemistry·2026
Same author

Flow injection amperometric sensor for sulfite detection in worldwide noodles using a novel redox composite of nickel hexacyanoferrate decorated on 3D mesoporous graphene aerogel.

Talanta·2025
Same author

A metal-free nanocomposite of polyaniline-decorated S-doped graphitic carbon nitride nanosheets for a flexible and disposable carbendazim sensor in fruit samples.

Food chemistry·2025
Same author

Sediment accumulation rate assessments for a large reservoir via <sup>210</sup>Pb and bulk density profiles.

The Science of the total environment·2025

Related Experiment Video

Updated: Mar 3, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K

Capacitive antibacterial susceptibility screening test with a simple renewable sensing surface.

Saroh Niyomdecha1, Warakorn Limbut2, Apon Numnuam1

  • 1Trace Analysis and Biosensor Research Center, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Center of Excellence for Innovation in Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand; Department of Chemistry, Faculty of Science, Prince of Songkla University, Hat Yai, Songkhla 90112, Thailand.

Biosensors & Bioelectronics
|May 3, 2017
PubMed
Summary

A novel renewable surface using 3-aminophenylboronic acid (3-APBA) enables rapid antibacterial susceptibility testing in 2.5 hours. This reusable sensor accurately detects bacterial response to cell-wall-targeting agents, offering a significant advancement in antimicrobial testing.

Keywords:
3-aminophynylboronic acid (3-APBA)Antibacterial susceptibilityCapacitive sensor

More Related Videos

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
06:32

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Published on: May 2, 2025

864
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

25.2K

Related Experiment Videos

Last Updated: Mar 3, 2026

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor
08:22

Ultrasensitive Detection of Biomarkers by Using a Molecular Imprinting Based Capacitive Biosensor

Published on: February 16, 2018

12.7K
Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor
06:32

Dynamic Multiparameter Platelet Function Assessment Using a Capacitive Biosensor

Published on: May 2, 2025

864
Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

25.2K

Area of Science:

  • Biotechnology
  • Analytical Chemistry
  • Microbiology

Background:

  • Antibacterial susceptibility testing is crucial for guiding antibiotic treatment.
  • Current methods can be time-consuming, delaying effective patient care.
  • Developing rapid and reusable diagnostic tools is a key challenge in infectious disease management.

Purpose of the Study:

  • To develop a simple, renewable surface for rapid antibacterial susceptibility testing.
  • To demonstrate the efficacy of a 3-aminophenylboronic acid (3-APBA) modified electrode for bacterial detection.
  • To establish a fast and repeatable method for assessing bacterial susceptibility to antimicrobial agents.

Main Methods:

  • Modification of an electrode surface with 3-aminophenylboronic acid (3-APBA) to bind bacterial cell wall cis-diol groups.
  • Utilizing a capacitive system for rapid detection of bacterial binding.
  • Employing an acid solution wash to regenerate the electrode surface for reuse.
  • Validation using scanning electron microscopy (SEM) and comparison with literature data.

Main Results:

  • The 3-APBA modified electrode demonstrated binding with both gram-positive and gram-negative bacteria.
  • The entire testing process, including regeneration, was completed in 2.5 hours.
  • The electrode showed good repeatability and could be reused up to 35 times.
  • Sensor results correlated well with established antimicrobial data and SEM observations.

Conclusions:

  • A renewable electrode surface provides a rapid and efficient platform for antibacterial susceptibility testing.
  • The sensor is effective for evaluating susceptibility to agents targeting bacterial cell walls.
  • This technology has potential for testing natural products and offers a faster alternative to conventional methods.