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Temporal focusing-based widefield multiphoton microscopy with spatially modulated illumination for biotissue imaging

Chia-Yuan Chang1,2, Cheng-Han Lin2, Chun-Yu Lin3

  • 1Center for Micro/Nano Science and Technology, National Cheng Kung University, 701, Tainan, Taiwan.

Journal of Biophotonics
|May 3, 2017
PubMed
Summary

This study developed a temporal focusing multiphoton excitation microscope (TFMPEM) with patterned illumination. The enhanced microscope improves axial excitation confinement and image contrast for biotissue imaging.

Keywords:
multiphoton processesnonlinear microscopythree-dimensional microscopy

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