Related Experiment Video

Updated: Mar 3, 2026

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis
11:44

Analysis of Combinatorial miRNA Treatments to Regulate Cell Cycle and Angiogenesis

Published on: March 30, 2019

8.1K

MicroRNA-497 suppresses renal cell carcinoma by targeting VEGFR-2 in ACHN cells

Sun Pengcheng1, Wang Ziqi1, Yin Luyao1

  • 1Department of Urology, The Fourth Hospital of Harbin Medical University, Harbin 150001, China.

Bioscience Reports
|May 4, 2017
PubMed

Insights

MicroRNA-497 (miR-497) is downregulated in renal cell carcinoma (RCC). Restoring miR-497 inhibits RCC cell viability, migration, and invasion while promoting apoptosis by targeting VEGFR-2.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • MicroRNAs (miRNAs) play crucial roles in cancer development, influencing cell proliferation, apoptosis, and drug resistance.
  • Aberrant miRNA expression is implicated in various cancers, including renal cell carcinoma (RCC).

Purpose of the Study:

  • To investigate the role of miR-497 in renal cell carcinoma (RCC).
  • To elucidate the mechanism underlying miR-497's function in RCC, focusing on its potential targets and signaling pathways.

Main Methods:

  • Quantitative reverse transcription PCR (RT-PCR) to assess miR-497 expression in ccRCC tissues.
  • In vitro studies using ACHN cells treated with miR-497 mimics and inhibitors to evaluate effects on cell viability, apoptosis, migration, and invasion.
  • Western blotting (WB) and luciferase reporter assays to identify and validate downstream targets of miR-497.
  • Analysis of MEK/ERK and p38 MAPK signaling pathways.

Main Results:

  • miR-497 expression was significantly decreased in clear cell RCC (ccRCC) tissues compared to adjacent normal tissues.
  • Overexpression of miR-497 in ACHN cells inhibited cell viability, migration, and invasion, while promoting apoptosis.
  • VEGFR-2 was identified as a direct target of miR-497, with miR-497 binding to the 3'-UTR of VEGFR-2 and suppressing its expression.
  • miR-497 influenced the MEK/ERK and p38 MAPK signaling pathways.

Conclusions:

  • miR-497 acts as a tumor suppressor in renal cell carcinoma (RCC).
  • The tumor-suppressive effects of miR-497 in RCC are mediated through the direct targeting of VEGFR-2.
  • miR-497 may represent a potential therapeutic target for RCC treatment by modulating VEGFR-2 and associated signaling pathways.
Keywords:
MEK/ERKVEGFR-2ccRCCmiR-497p38 MAPK

More Related Videos

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K
MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues12:21

MicroRNA In situ Hybridization for Formalin Fixed Kidney Tissues

Published on: November 30, 2013

15.4K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
Experimental RNAi02:15

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...
8.1K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K