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Updated: Mar 3, 2026

Counting Proteins in Single Cells with Addressable Droplet Microarrays
12:25

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Published on: July 6, 2018

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High throughput single cell counting in droplet-based microfluidics.

Heng Lu1, Ouriel Caen1, Jeremy Vrignon2

  • 1INSERM UMR-S1147, CNRS SNC5014, Paris Descartes University, Equipe labellisée Ligue Nationale contre le cancer, Paris, France.

Scientific Reports
|May 4, 2017
PubMed
Summary
This summary is machine-generated.

Accurately count diverse cells using a novel microfluidic hemocytometer. This high-throughput method ensures robust data for single-cell studies and droplet-based experiments.

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Area of Science:

  • Microfluidics
  • Cell Biology
  • High-Throughput Screening

Background:

  • Droplet-based microfluidics is crucial for high-throughput single-cell analysis.
  • Accurate cell counting is essential for the reliability of these studies.

Purpose of the Study:

  • To develop a simple and precise method for accurate cell counting.
  • To enable high-throughput counting of diverse cell types, including adherent and non-adherent human cells and bacteria.

Main Methods:

  • Utilized a microfluidic hemocytometer for cell counting.
  • Applied the method to large populations of cells in a high-throughput manner.

Main Results:

  • Demonstrated accurate counting of adherent and non-adherent human cells.
  • Successfully counted bacteria with high precision.
  • Generated statistically relevant data for large cell populations.

Conclusions:

  • The microfluidic hemocytometer offers a robust solution for accurate cell quantification.
  • This method supports characterization of cell encapsulation and viability in droplet-based systems.