Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Yeast Signaling01:28

Yeast Signaling

18.3K
Yeasts are single-celled organisms, but unlike bacteria, they are eukaryotes (cells with a nucleus). Cell signaling in yeast is similar to signaling in other eukaryotic cells. A ligand, such as a protein or a small molecule released from a yeast cell, attaches to a receptor on the cell surface. The binding stimulates second-messenger kinases to activate or inactivate transcription factors that further regulate gene expression. Many of the yeast intracellular signaling cascades have similar...
18.3K
Diversity of Protists IV01:27

Diversity of Protists IV

1.5K
Amoebozoa represent a diverse group of terrestrial and aquatic protists that utilize lobe-shaped pseudopodia for locomotion and feeding. This characteristic differentiates them from the Rhizaria, which possess threadlike pseudopodia. The primary classifications within Amoebozoa include gymnamoebas, entamoebas, and the plasmodial and cellular slime molds. Phylogenetic evidence indicates that Amoebozoa diverged from a lineage that ultimately gave rise to fungi and animals.Gymnamoebas and...
1.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

NFYB-lncRNA axis resets the tumor microenvironment to promote HCC aggressive progression by a positive feedback loop.

Acta pharmaceutica Sinica. B·2026
Same author

Identification of a master regulator Msd1 that governs meiotic entry in a global basidiomycete pathogen.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

An immunogenomic classification of solid tumours reveals subtype-specific therapeutic vulnerabilities for immunotherapy.

EBioMedicine·2026
Same author

Mechanism and optimization of a Hg<sup>2+</sup>-triggered nanozyme based on chitosan-carbon quantum dots-tellurium nanoparticles.

International journal of biological macromolecules·2026
Same author

Separation of Human P63<sup>+</sup> Lung Progenitor Cells from Growth-Arrested Feeders Using Multidimensional Double Spiral Inertial Microfluidics for Efficient Bioprocessing.

Analytical chemistry·2026
Same author

Cryptococcus neoformans adapts to host CO2 concentrations via metabolic and stress-response remodeling.

PLoS biology·2026
Same journal

Genetic survey of biomarkers at early and mid-pregnancy identifies pregnancy-specialized immune regulation.

PLoS genetics·2026
Same journal

Argonaute proteins orchestrate Meiotic Sex Chromosome Inactivation and timing of the spermatogenic transcriptional program.

PLoS genetics·2026
Same journal

Genome wide association study meta-analysis of neuropathologic lesions of Alzheimer's disease and related dementias in a multi-site autopsy cohort.

PLoS genetics·2026
Same journal

Microtubule stiffening by the doublecortin-domain protein ZYG-8 contributes to mitotic spindle orientation during zygote division in Caenorhabditis elegans.

PLoS genetics·2026
Same journal

Multiple instance fine-mapping: Predicting causal regulatory variants with a deep sequence model.

PLoS genetics·2026
Same journal

Nuclear ubiquitin-conjugating enzyme TrUbc4 and F-box protein TrFwd1-mediated modification of Cre1 in Trichoderma reesei establishes a regulatory mechanism for carbon catabolite repression.

PLoS genetics·2026
See all related articles

Related Experiment Video

Updated: Mar 3, 2026

Microscopy of Fission Yeast Sexual Lifecycle
07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

15.3K

Pheromone independent unisexual development in Cryptococcus neoformans.

Rachana Gyawali1, Youbao Zhao1, Jianfeng Lin1

  • 1Department of Biology, Texas A&M University, College Station, United States of America.

Plos Genetics
|May 4, 2017
PubMed
Summary
This summary is machine-generated.

In Cryptococcus neoformans, a novel condition suppresses bisexual reproduction, allowing α mating type to dominate via enhanced unisexual reproduction. The pheromone pathway, usually key for mating, is surprisingly inhibitory under these conditions.

More Related Videos

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
05:34

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus

Published on: June 6, 2025

648
Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior
06:49

Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior

Published on: August 9, 2024

3.4K

Related Experiment Videos

Last Updated: Mar 3, 2026

Microscopy of Fission Yeast Sexual Lifecycle
07:47

Microscopy of Fission Yeast Sexual Lifecycle

Published on: March 9, 2016

15.3K
Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus
05:34

Sexual Crosses with the Mucoromycete Phycomyces blakesleeanus

Published on: June 6, 2025

648
Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior
06:49

Author Spotlight: Examining Volatile Sex Pheromone Influence on Male C. elegans Behavior

Published on: August 9, 2024

3.4K

Area of Science:

  • Mycology
  • Fungal Genetics
  • Reproductive Biology

Background:

  • Cryptococcus neoformans exhibits both bisexual (a-α) and unisexual reproduction, both requiring meiosis.
  • While bisexual reproduction is efficient in labs, the α mating type predominates in nature, suggesting a role for unisexual reproduction.
  • The dominance of the α mating type and inefficient lab-observed unisexual reproduction present a scientific puzzle.

Purpose of the Study:

  • To investigate the conditions governing the balance between bisexual and unisexual reproduction in Cryptococcus neoformans.
  • To understand the role of the pheromone pathway in regulating these distinct reproductive modes.
  • To identify genetic factors controlling unisexual reproduction.

Main Methods:

  • Comparative analysis of reproductive strategies under varying conditions.
  • Genetic manipulation, including insertional mutagenesis of the mat2 gene.
  • Transcriptome analysis to identify key regulatory genes.
  • Investigating the roles of Znf2 and calcineurin in sexual development.

Main Results:

  • A previously unknown condition was identified that promotes unisexual reproduction while inhibiting bisexual reproduction.
  • The pheromone pathway, including Mat2, is inhibitory for unisexual reproduction under these specific conditions.
  • Calcineurin and Znf2 were found to be essential and cooperative regulators of unisexual development.
  • Mat2 functions bifunctionally, repressing unisexual development and activating bisexual development.

Conclusions:

  • The findings reveal a regulatory switch for sexual reproduction modes in Cryptococcus neoformans.
  • Mat2's dual role as an activator and repressor explains the skewed mating type distribution in nature.
  • This discovery sheds light on the complex evolutionary strategies of this ubiquitous fungus.