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Immunoregulatory effects of catrix.
J Rosen1, W T Sherman, J F Prudden
1Department of Pathology, New York University Medical Center, New York.
Journal of Biological Response Modifiers
|October 1, 1988
Summary
Catrix-S, a soluble derivative of an acidic mucopolysaccharide complex, enhances both T-dependent and T-independent antibody production in mice. Its immunoaugmenting activity is linked to chondroitin sulfate and may involve direct B cell effects or macrophage mediation.
Area of Science:
- Immunology
- Biochemistry
Background:
- Catrix is an acidic mucopolysaccharide complex containing glycosaminoglycans, including chondroitin sulfate.
- Understanding the immunoregulatory effects of such compounds is crucial for developing immune-modulating therapies.
Purpose of the Study:
- To investigate the immunoregulatory effects of Catrix, specifically its soluble derivative Catrix-S, on antibody production in vitro and in vivo.
- To determine the role of chondroitin sulfate in Catrix-S's immunoaugmenting activity.
Main Methods:
- Mice were used to examine in vitro and in vivo antibody production following administration of Catrix-S.
- Dose-response studies were conducted using intraperitoneal and intravenous administration.
- In vitro proliferative responses were assessed, and the effects of chondroitinase ABC, dextran sulfate, lipopolysaccharide, and chondroitin sulfate C were evaluated.
- Fractionation techniques, including ammonium sulfate precipitation and molecular weight separation, were employed to isolate active components.
Main Results:
- Catrix-S enhanced T-dependent and T-independent antibody responses in vivo in a dose-dependent manner, with optimal effects at specific doses.
- The immunoaugmenting activity was found to be related to the chondroitin sulfate component, as indicated by similar effects and inactivation by chondroitinase ABC.
- In vitro, Catrix-S showed additive effects with dextran sulfate and lipopolysaccharide but not chondroitin sulfate C on proliferative responses.
- Separation of inhibitory and stimulatory activities was achieved through fractionation, with the immunoaugmenting effect found in specific ammonium sulfate precipitates and molecular weight fractions.
Conclusions:
- Catrix-S exhibits significant immunoaugmenting properties, enhancing antibody production through mechanisms potentially involving direct B cell effects and/or indirect modulation of macrophages.
- The chondroitin sulfate component of Catrix-S is critical for its stimulatory effects on the immune system.
- The study highlights the potential of Catrix-S as an immune modulator, with distinct stimulatory and inhibitory activities that can be separated.