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Manipulation and Analysis of Cell Cycle-Dependent Processes in Budding Yeast
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A Slowed Cell Cycle Stabilizes the Budding Yeast Genome.

Peter J Vinton1, Ted Weinert2

  • 1Department of Molecular and Cellular Biology, University of Arizona, Tucson, Arizona 85721.

Genetics
|May 5, 2017
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Summary

Slowing the cell cycle, independent of checkpoints, stabilizes the genome by allowing more time for DNA error correction. This "time checkpoint" benefits chromosome stability, even for errors that don't trigger normal checkpoint responses.

Keywords:
accuracychromosome instabilitydelayed cell cycleerv14speed

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Area of Science:

  • Cell Biology
  • Genetics
  • Molecular Biology

Background:

  • Cell cycle checkpoints regulate DNA replication and repair during cell division.
  • Genome stability is crucial for preventing mutations and ensuring accurate cell division.
  • It is hypothesized that a slower cell cycle may inherently improve genome stability, independent of checkpoint activation.

Purpose of the Study:

  • To test if a slowed cell cycle stabilizes the genome independently of checkpoints in yeast.
  • To investigate the role of the gene ERV14 in genome stability and cell cycle regulation.

Main Methods:

  • Utilized budding yeast Saccharomyces cerevisiae.
  • Employed three different chromosome assays to measure genome stability.
  • Investigated mutations in ERV14 and other genetic/chemical treatments to induce cell cycle delays.

Main Results:

  • Mutation of ERV14 unexpectedly stabilized the genome.
  • A slowed cell cycle, induced by ERV14 mutation or other means, correlated with increased genome stability.
  • This stabilization effect was observed even in checkpoint-proficient cells, suggesting a checkpoint-independent mechanism.

Conclusions:

  • A slowed cell cycle provides a "time checkpoint" that enhances genome stability.
  • This mechanism allows for more frequent or effective DNA error correction, particularly for errors that may not activate canonical checkpoints.
  • A delay in the G2/M phase of the cell cycle appears to commonly promote genome stabilization.