Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.5K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.5K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.7K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

FLOWR.ROOT - A flow matching-based foundation model for joint multi-purpose structure-aware 3D ligand generation and affinity prediction.

Nature communications·2026
Same author

Corrigendum to "Isolation and characterization of chitinolytic fast-growing bacteria from marine environment in Vietnam". [J. Genet. Eng. Biotechnol. 23 (2025) 100587].

Journal, genetic engineering & biotechnology·2026
Same author

Speckle-based measurement of the fractional azimuthal index of orbital angular momentum beams for refractive index sensing.

Nature communications·2026
Same author

Circulating tumor cell detection in cancer patients using in-flow deep learning holography.

Npj biosensing·2026
Same author

Superconducting nanowire single-photon detectors for enhanced biomedical imaging.

Journal of biomedical optics·2026
Same author

Quantitative Assessment of Myocardial Infarction Scarring using Optical Coherence Tomography: towards data-driven Catheter Therapy Guidance.

IEEE transactions on bio-medical engineering·2026
Same journal

Peripheral B-cell receptor repertoire predicts immune-related adverse events following immune checkpoint inhibitor therapy in advanced renal cell carcinoma.

Scientific reports·2026
Same journal

Effects of black soldier fly (Hermetia illucens L.) larvae zoocompost on the mineral element content of blue honeysuckle berries.

Scientific reports·2026
Same journal

Investigation on absorption refrigeration performance of R1243zf with imidazolium ionic liquid as the working pairs.

Scientific reports·2026
Same journal

DeepTriage-CN: integrating clinical text with vital signs for emergency department admission prediction in an aging population.

Scientific reports·2026
Same journal

Gold nanoparticles as dual-action antiviral agents: disruption of SARS-CoV-2 viral envelopes and RNA integrity.

Scientific reports·2026
Same journal

Comparison of capillary microsampling and venous blood for multi-pathogen serosurveillance.

Scientific reports·2026
See all related articles

Related Experiment Video

Updated: Mar 3, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K

Integrated single- and two-photon light sheet microscopy using accelerating beams.

Peeter Piksarv1,2, Dominik Marti3, Tuan Le4

  • 1SUPA, School of Physics and Astronomy, University of St Andrews, North Haugh, St Andrews, KY16 9SS, UK.

Scientific Reports
|May 5, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces the first light sheet microscope utilizing Airy beams, enabling compact, high-resolution imaging without scanning. This novel approach significantly enhances the field of view in two-photon microscopy for biomedical research.

More Related Videos

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.5K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.6K

Related Experiment Videos

Last Updated: Mar 3, 2026

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope
08:53

Single Plane Illumination Module and Micro-capillary Approach for a Wide-field Microscope

Published on: August 15, 2014

10.2K
Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy
08:32

Author Spotlight: Advancing Knowledge in Far-From-Equilibrium Materials Through Light-Sheet Microscopy

Published on: January 26, 2024

3.5K
Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers
10:07

Highly Resolved Intravital Striped-illumination Microscopy of Germinal Centers

Published on: April 9, 2014

10.6K

Area of Science:

  • Biomedical Optics
  • Microscopy Technology
  • Neuroscience Imaging

Background:

  • Light sheet microscopy is a powerful technique for biological imaging.
  • Traditional light sheet systems often require beam scanning, increasing complexity and size.
  • Gaussian beams are commonly used, but have limitations in field of view.

Purpose of the Study:

  • To demonstrate a novel light sheet microscope design.
  • To utilize propagation-invariant, accelerating Airy beams for enhanced imaging.
  • To enable both single- and two-photon imaging modes in a compact system.

Main Methods:

  • Development of a light sheet microscope employing Airy beams.
  • Operation in both single-photon and two-photon excitation modes.
  • Comparison with standard Gaussian beam illumination.

Main Results:

  • Achieved an ultra-compact, high-resolution light sheet system without the need for beam scanning.
  • Demonstrated a six-fold increase in the field of view in two-photon mode compared to Gaussian beams.
  • Validated the system's capability for advanced biomedical imaging.

Conclusions:

  • Airy beam-based light sheet microscopy offers significant advantages in size and field of view.
  • This technology opens new avenues for studying neuronal processes and other biomedical applications.
  • The developed system represents a breakthrough in high-resolution, wide-field biological imaging.