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Related Experiment Video

Updated: Mar 3, 2026

CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
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Automated multiplex genome-scale engineering in yeast.

Tong Si1,2, Ran Chao1,2, Yuhao Min3

  • 1Carl R. Woese Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Nature Communications
|May 5, 2017
PubMed
Summary
This summary is machine-generated.

We developed an automated platform for genome-scale engineering in yeast (Saccharomyces cerevisiae). This system enables rapid functional mapping and optimization of yeast genes for various industrial applications.

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Area of Science:

  • Synthetic Biology
  • Microbial Engineering
  • Genomics

Background:

  • Genome-scale engineering is crucial for understanding and modifying microorganisms.
  • Current tools are primarily limited to bacterial systems, hindering eukaryotic applications.

Purpose of the Study:

  • To develop an automated platform for multiplex genome-scale engineering in Saccharomyces cerevisiae.
  • To enable high-throughput functional mapping and optimization of yeast genes.

Main Methods:

  • Creation of standardized genetic parts for >90% of yeast genes (overexpression and knockdown).
  • Iterative, modular integration of genetic parts into repetitive genomic sequences using CRISPR-Cas and robotic automation.

Main Results:

  • Demonstrated functional mapping and multiplex optimization for diverse phenotypes.
  • Successfully engineered yeast for improved cellulase expression, isobutanol production, glycerol utilization, and acetic acid tolerance.

Conclusions:

  • The automated platform significantly accelerates genome-scale engineering in yeast.
  • This technology is valuable for yeast-based microbial cell factories and fundamental research.