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The relationship between amniotic fluid miRNAs and congenital obstructive nephropathy

Juntao Xie1, Yi Zhou2, Wenzong Gao1

  • 1Department of Pediatric Surgery, The First Affiliated Hospital, Sun Yat-sen UniversityGuangdong, People's Republic of China.

American Journal of Translational Research
|May 5, 2017
PubMed

Insights

Exosomal microRNAs (miRNAs) in amniotic fluid show promise as biomarkers for prenatal diagnosis of congenital hydronephrosis. Specific miRNAs, like hsa-miR-300 and hsa-miR-299-5p, may indicate kidney fibrosis.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Genetics

Background:

  • Exosomes, small vesicles in bodily fluids, contain proteins and RNA.
  • Exosomal contents, including microRNAs (miRNAs), are potential biomarkers for diagnostics.

Purpose of the Study:

  • To investigate exosomal miRNAs in amniotic fluid as biomarkers for prenatal diagnosis of congenital hydronephrosis.
  • To evaluate fetal kidney function using exosomal miRNA profiles.

Main Methods:

  • Exosomes isolated from amniotic fluid of fetuses with and without congenital hydronephrosis.
  • Transmission electron microscopy, flow cytometry, and western blot for exosome identification (CD24, CD9 expression).
  • Microarray analysis of exosomal miRNA, followed by qPCR validation and bioinformatic target analysis (KEGG pathways).

Main Results:

  • Four miRNAs (has-miR-942, has-miR-4289, has-miRPlus-A1073, has-miR-195-3p) were upregulated; 35 miRNAs were downregulated in congenital hydronephrosis cases.
  • hsa-miR-300 and hsa-miR-299-5p target genes are involved in the Wnt signaling pathway.
  • Specific target genes (DVL2, PP2R5A, SRFP2, SIAH1) identified for hsa-miR-300 and hsa-miR-299-5p.

Conclusions:

  • Exosomal miRNAs in amniotic fluid can serve as potential biomarkers for prenatal diagnosis of congenital hydronephrosis.
  • Reduced expression of hsa-miR-300 and hsa-miR-299-5p may indicate kidney fibrosis in congenital obstructive nephropathy.
Keywords:
Congenital obstructive nephropathyexosomemiRNAprenatal diagnosis

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