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Monitoring Protein Adsorption with Solid-state Nanopores
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Solid-state nanopore analysis of alcohol-soluble molecules.

Dhrubajyoti Basu Roy1, Adam R Hall

  • 1Virginia Tech-Wake Forest School of Biomedical Engin and Sciences, Wake Forest University School of Medicine, Winston-Salem, NC 27101, USA.

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We developed a new nanopore method to detect alcohol-soluble proteins using ethanol-water mixtures. This technique successfully measured zeins, a type of maize protein, demonstrating its potential for protein analysis.

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Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Materials Science

Background:

  • Solid-state nanopore sensing is a powerful tool for molecule detection.
  • Analyzing alcohol-soluble proteins presents challenges due to solvent limitations.

Purpose of the Study:

  • To develop and validate a modified solid-state nanopore measurement scheme for probing alcohol-soluble proteins.
  • To demonstrate the feasibility of operating nanopore devices in ethanol-water mixtures.

Main Methods:

  • Utilized lithium chloride (LiCl) as an electrolyte, leveraging its alcohol solubility.
  • Operated nanopore devices in azeotropic mixtures of ethanol and water.
  • Characterized nanopore conductivity as a function of ethanol content, nanopore diameter, and salt concentration.
  • Employed resistive-pulse sensing to measure electrical translocations of proteins.

Main Results:

  • Established ionic response of nanopores in ethanol-water mixtures, consistent with established models.
  • Successfully measured electrical translocations of zeins, a class of alcohol-soluble maize proteins.
  • Demonstrated the utility of the modified scheme for analyzing specific protein types.

Conclusions:

  • The modified solid-state nanopore scheme is effective for detecting alcohol-soluble proteins.
  • Operation in ethanol-water mixtures expands the applicability of nanopore sensing.
  • This method offers a novel approach for characterizing proteins like zeins.