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A Lab-On-A-Chip Platform for Stimulating Osteocyte Mechanotransduction and Analyzing Functional Outcomes of Bone Remodeling
Published on: May 21, 2020
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In-vivo evaluation of subcutaneously implanted cell-loaded apatite microcarriers for osteogenic potency
Poon Nian Lim1, Jason Feng1, Zuyong Wang1,2
1Department of Mechanical Engineering, National University of Singapore, Singapore, 117 576, Singapore.
Summary
Human foetal mesenchymal stem cells (hfMSC)-loaded apatite microcarriers effectively promote ectopic bone formation. These cell-loaded microcarriers show retained osteogenic potency, offering a promising platform for bone regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Medicine
- Stem Cell Biology
Background:
- Apatite microcarriers are explored as scaffolds for in-vivo cell delivery to enhance bone regeneration.
- Evaluating the osteogenic potential of cell-loaded scaffolds is crucial for regenerative therapies.
Purpose of the Study:
- To assess the osteogenic capacity of human foetal mesenchymal stem cells (hfMSC)-loaded apatite microcarriers in a subcutaneous mouse model.
- To investigate ectopic bone formation and tissue remodeling at different time points post-implantation.
Main Methods:
- Subcutaneous implantation of hfMSC-loaded apatite microcarriers in mice.
- Histological analysis using H&E, Masson's trichrome, and Von Kossa staining.
- Immunohistochemistry for osteopontin to confirm active bone formation.
Main Results:
- Significant ectopic bone formation and tissue organization observed in hfMSC-loaded microcarrier groups.
- Evidence of active bone remodeling, increased tissue density, and mineralization at 2 months.
- Absence of ectopic bone formation in control groups with empty microcarriers.
Conclusions:
- hfMSC-loaded apatite microcarriers retain osteogenic potency after in-vivo implantation.
- These microcarriers serve as an effective platform for promoting bone tissue regeneration.
- The study demonstrates successful ectopic bone formation, validating the scaffold's potential.
