Drug loaded microbeads entrapped electrospun mat for wound dressing application

Area of Science:

• Biomaterials Science
• Drug Delivery Systems
• Wound Healing Technologies

Background:

• Developing advanced wound dressings is crucial for infection control and promoting healing.
• Antibiotic-loaded materials offer localized drug delivery to combat wound infections.
• Chitosan and polycaprolactone are biocompatible polymers with potential in biomedical applications.

Purpose of the Study:

• To design and evaluate a novel antibiotic-loaded wound dressing.
• To assess the in vitro biocompatibility and antimicrobial efficacy of the developed material.
• To explore the potential for multi-drug delivery in future iterations.

Main Methods:

• Fabrication of ampicillin-loaded chitosan microbeads within a polycaprolactone electrospun mat (MbAPPCL).
• Morphological analysis using scanning electron microscopy (SEM) and surface chemistry by Fourier Transform Infrared Spectroscopy (FTIR).
• In vitro cytotoxicity (L-929 fibroblast cells), cell adhesion/viability, hemocompatibility (hemolysis, platelet adhesion), and antibiotic susceptibility (Staphylococcus aureus) tests.

Main Results:

• SEM and FTIR confirmed the successful fabrication and structure of MbAPPCL.
• In vitro tests demonstrated non-cytotoxicity and excellent cytocompatibility of MbAPPCL.
• Hemocompatibility was confirmed through low hemolysis and platelet adhesion.
• Antibiotic susceptibility tests showed effective drug release and antimicrobial activity against Staphylococcus aureus.

Conclusions:

• The developed MbAPPCL wound dressing exhibits promising in vitro biocompatibility and antimicrobial properties.
• This design provides a foundation for advanced wound management strategies.
• The platform allows for the incorporation of various antibiotics for multi-drug therapeutic approaches.