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Ferroptosis and Cell Death Analysis by Flow Cytometry.

Daishi Chen1, Ilker Y Eyupoglu1, Nicolai Savaskan2,3

  • 1Translational Cell Biology and Neurooncology Laboratory of the Universitätsklinikum Erlangen (UKER), Friedrich-Alexander University of Erlangen - Nürnberg (FAU), and Department of Neurosurgery of the Universitätsklinikum Erlangen, Universitätsklinikum Erlangen (UKER), Friedrich-Alexander University of Erlangen - Nürnberg (FAU), Schwabachanlage 6, Erlangen 91054, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|May 5, 2017
PubMed
Summary

This study presents a flow cytometry method to identify and quantify cell death, including the regulated form ferroptosis. This technique is crucial for accurate data in biological and clinical research.

Keywords:
ErastinFerroptosisFluorescence-activated cell sortingNecrosis ApoptosisNeurodegenerationNeurooncologySorafenibTumorsxCT

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Area of Science:

  • Cell biology
  • Biochemistry
  • Immunology

Background:

  • Cell death, including regulated ferroptosis, has distinct characteristics and is implicated in various diseases.
  • Accurate identification of cell death is vital for experimental integrity and clinical applications.
  • Flow cytometry is a key technique for cell analysis, but dead cells can interfere with results.

Purpose of the Study:

  • To describe a standard flow cytometry procedure for detecting and quantifying cell death.
  • To specifically address the identification and measurement of ferroptosis using flow cytometry.
  • To ensure robust and reproducible identification of dead cells in cytometry experiments.

Main Methods:

  • Utilizing standard flow cytometry techniques.
  • Developing a procedure to distinguish between viable and dead cells.
  • Implementing methods to specifically detect ferroptosis.

Main Results:

  • A reliable procedure for cell death detection via flow cytometry was established.
  • The method allows for the quantification of ferroptosis.
  • The technique ensures reproducible identification of dead cells, improving data quality.

Conclusions:

  • The described flow cytometry procedure effectively detects and quantifies cell death and ferroptosis.
  • This method enhances the reliability of cell analysis in research and clinical settings.
  • Accurate cell death characterization is essential for understanding pathological conditions and drug effects.