Related Experiment Videos
Mammalian ets-1 and ets-2 genes encode highly conserved proteins.
D K Watson1, M J McWilliams, P Lapis
1Laboratory of Molecular Oncology, National Cancer Institute, Frederick, MD 21701-1013.
Summary
The ETS1 and ETS2 genes in mammals are highly conserved, homologous to avian leukemia virus E26. Sequencing human and mouse cDNA clones revealed their structures and protein-coding regions, confirming ETS as a gene family.
Area of Science:
- Genetics
- Molecular Biology
- Oncology
Background:
- Cellular ets sequences, homologous to the v-ets sequence of avian leukemia virus E26, are highly conserved across species.
- In mammals, these ets sequences are located at two distinct chromosomal loci: ets-1 and ets-2.
Purpose of the Study:
- To elucidate the gene structure of human ETS1 and ETS2.
- To identify the open reading frames (ORFs) encoding putative proteins for these genes.
- To compare human and mouse ets-2 cDNA clones.
Main Methods:
- Sequencing of human ets-1 cDNA clones.
- Sequencing of human and mouse ets-2 cDNA clones.
- Bioinformatic analysis to identify ORFs and compare protein sequences.
Main Results:
- The human ETS1 gene encodes a 441-amino acid protein, over 95% identical to the chicken c-ets-1 gene product.
- Human and mouse ets-2 cDNA clones are closely related, encoding proteins of 469 and 468 residues, respectively.
- The ets gene family members share distinct conserved domains.
Conclusions:
- The human ETS1 gene is homologous to the chicken c-ets-1 protooncogene.
- The findings confirm ets as a gene family with conserved domains.
- This research provides foundational structural information for the ETS gene family in mammals.