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A simple and efficient non-denaturing FISH method for maize chromosome differentiation using single-strand
Minqiu Zhu1, Pei Du1, Lifang Zhuang1
1a Agriculture College, Nanjing Agricultural University, Nanjing 210095, China.
Genome
|May 5, 2017
Summary
New single-strand oligonucleotide (SSON) probes enable precise maize chromosome identification. A multiplex probe set effectively distinguishes genotypes, aiding maize genetic research and breeding.
Area of Science:
- Plant genetics
- Cytogenetics
- Molecular biology
Background:
- Single-strand oligonucleotides (SSONs) are increasingly used for chromosome painting across species.
- Accurate chromosome identification is crucial for understanding plant genetics and breeding.
Purpose of the Study:
- To develop and evaluate SSONs for maize (Zea mays L.) chromosome identification.
- To create a multiplex probe set for efficient characterization of maize chromosomes and genetic variation.
Main Methods:
- Development and application of nine SSONs for chromosome identification in maize.
- Utilizing non-denaturing fluorescence in situ hybridization (ND-FISH) for probe analysis.
- Testing a multiplex probe set comprising four SSONs on 16 maize inbred lines and hybrids.
Main Results:
- Each SSON generated clear signals on 2-10 chromosomes in maize inbred lines B73 and Mo17.
- A multiplex probe set successfully identified all maize chromosomes in a single ND-FISH round.
- Unique signals on chromosome 6 differentiated all 16 maize genotypes, revealing chromosomal-level genetic variation.
Conclusions:
- Developed SSONs and a multiplex probe set are effective tools for maize chromosome identification.
- This approach facilitates genotype identification and advances chromosome research in maize.
- The findings support the use of SSONs for high-throughput genetic analysis in maize breeding programs.
