Related Experiment Video

Updated: Mar 3, 2026

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells
09:45

An In Vitro Protocol for Evaluating MicroRNA Levels, Functions, and Associated Target Genes in Tumor Cells

Published on: May 21, 2019

9.0K

MicroRNA-524-5p Functions as a Tumor Suppressor in a Human Pituitary Tumor-Derived Cell Line

Wang Zhen1,2, Du Qiu1,2, Chen Zhiyong3

  • 1Department of Histology and Embryology, Medical school of Sun Yat-sen University, Guangzhou, Guangdong, China.

Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Metabolisme
|May 5, 2017
PubMed

Insights

MicroRNA-524-5p (miR-524-5p) inhibits nonfunctional pituitary adenoma (NFA) cell growth and spread. Enhancing miR-524-5p offers a potential therapeutic strategy for NFAs.

Area of Science:

  • Endocrinology
  • Molecular Biology
  • Oncology

Background:

  • Nonfunctional pituitary adenomas (NFAs) represent a significant portion of pituitary tumors, often lacking distinct hormonal symptoms.
  • MicroRNAs are increasingly recognized for their roles in pituitary adenoma development and progression.
  • The specific function of microRNA-524-5p (miR-524-5p) in NFAs has not been previously elucidated.

Purpose of the Study:

  • To investigate the functional impact of miR-524-5p overexpression on pituitary-derived folliculostellate (PDFS) cells.
  • To assess the effects of miR-524-5p on cell proliferation, migration, invasion, and tumorigenicity in vitro and in vivo.

Main Methods:

  • Utilized lentiviral transfection to overexpress miR-524-5p in PDFS cells.
  • Evaluated changes in pituitary tumor-transforming gene 1 (PTTG1) binding factor (PBF) expression at mRNA and protein levels.
  • Assessed cell proliferation, clonogenicity, migration, and invasion in vitro.
  • Investigated tumor growth inhibition in a nude mouse xenograft model.

Main Results:

  • Overexpression of miR-524-5p led to a significant downregulation of PBF expression.
  • miR-524-5p significantly attenuated PDFS cell proliferation, clonogenicity, migration, and invasion in vitro.
  • Enhanced miR-524-5p expression effectively inhibited tumor growth in vivo.

Conclusions:

  • miR-524-5p plays a crucial role in regulating the biological behavior of PDFS cells.
  • miR-524-5p demonstrates potential as a therapeutic target for nonfunctional pituitary adenomas.

More Related Videos

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library08:40

Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library

Published on: April 6, 2012

18.1K
In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.8K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.2K
The Retinoblastoma Gene01:20

The Retinoblastoma Gene

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
The first-ever tumor suppressor gene called Rb was identified in retinoblastoma - a rare eye tumor in children. In inherited forms of the disease, a child inherits one defective copy of the Rb gene, which predisposes them to retinoblastoma. However,...
4.8K
Loss of Tumor Suppressor Gene Functions01:12

Loss of Tumor Suppressor Gene Functions

Tumor suppressor genes are normal genes that can slow down cell division, repair DNA mistakes, or program the cells for apoptosis in case of irreparable damage. Hence, they play an essential role in preventing the proliferation of damaged cells.
When the tumor suppressor genes develop mutations or are lost, cells start growing out of control, leading to cancer. However, a single functional copy of the tumor suppressor gene is enough for the cells to maintain their normal functions and cell...
6.2K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.9K