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Related Experiment Video

Updated: Mar 3, 2026

Patch Clamp Recording of Starburst Amacrine Cells in a Flat-mount Preparation of Deafferentated Mouse Retina
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Multiple cell types form the VIP amacrine cell population.

Luis Pérez de Sevilla Müller1, Alexander Solomon1, Kristopher Sheets1

  • 1Department of Neurobiology, David Geffen School of Medicine at Los Angeles, University of California at Los Angeles, Los Angeles, California, 90095-1763.

The Journal of Comparative Neurology
|May 5, 2017
PubMed
Summary
This summary is machine-generated.

This study characterizes vasoactive intestinal peptide (VIP) amacrine cells in the mouse retina, revealing multiple subtypes with distinct morphologies and potential roles in visual processing.

Keywords:
AB_10058149Confetti (Brainbow2.1)GABARRID: AB_10013222RRID: AB_11214092RRID: AB_2187981RRID: AB_2201526RRID: AB_2209751RRID: AB_2395389RRID: AB_477483RRID: AB_477652RRID: AB_90764VIP-ires-Cre recombinaseamacrine cellsmouseretinavasoactive intestinal peptidevision

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Area of Science:

  • Neuroscience
  • Retinal Biology
  • Cellular Neuroscience

Background:

  • Amacrine cells are crucial interneurons in the inner retina, involved in visual information processing.
  • Vasoactive intestinal peptide (VIP) is a neuropeptide found in the retina, but its specific amacrine cell populations and functions are not fully understood.

Purpose of the Study:

  • To characterize the morphology, neurochemistry, and cell types of VIP-expressing amacrine cells in the mouse retina.
  • To identify distinct subtypes within the VIP-ires-Cre amacrine cell population.

Main Methods:

  • Generation of VIP-ires-Cre reporter mouse lines (tdTomato and Brainbow2.1).
  • Quantitative analysis, immunohistochemistry, and intracellular labeling of retinal sections and whole-mounts.
  • Evaluation of cell density, process ramification, and marker expression (Syntaxin 1A, GABA, VIP).

Main Results:

  • VIP-tdTomato and -Confetti cells were predominantly located in the inner nuclear layer (INL) and ganglion cell layer (GCL).
  • Processes ramified in specific strata (1, 3, 4, 5) of the inner plexiform layer (IPL).
  • Identified three main VIP amacrine cell types (VIP-1, VIP-2A/2B, VIP-3) with distinct stratification patterns and fields.

Conclusions:

  • VIP-ires-Cre amacrine cells constitute a heterogeneous, neuropeptide-expressing population in the retina.
  • These distinct VIP amacrine cell subtypes likely play specialized roles in visual processing within the IPL microcircuits.