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Updated: Mar 3, 2026

07:18
Highly-Multiplexed Tissue Imaging with Raman Dyes
Published on: April 21, 2022
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Stimulated Raman scattering of polymer nanoparticles for multiplexed live-cell imaging
Fanghao Hu1, Spencer D Brucks, Tristan H Lambert
1Department of Chemistry, Columbia University, 3000 Broadway, New York, NY 10027, USA. lcampos@columbia.edu wm2256@columbia.edu.
Abstract:
A novel nanoparticle-based imaging strategy is introduced that couples biocompatible organic nanoparticles and stimulated Raman scattering (SRS) microscopy. Polymer nanoparticles with vibrational labels incorporated were readily prepared for multi-color SRS imaging with excellent photo-stability. The Raman-active polymer dots are nontoxic, rapidly enter various cell types, and are applied in multiplexed cell-type sorting.

