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N-glycan in cockroach allergen regulates human basophil function.

Danh C Do1, Shuang Yang2, Xu Yao3

  • 1Division of Allergy and Clinical Immunology, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA.

Immunity, Inflammation and Disease
|May 6, 2017
PubMed
Summary

Cockroach allergen glycans play a key role in allergic reactions and asthma. These glycans, specifically those on Bla g 2, interact with basophils via DCIR, influencing immune responses.

Keywords:
GlycanC-type lectin receptorCockroach allergen

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Area of Science:

  • Immunology
  • Allergology
  • Glycobiology

Background:

  • Cockroach allergen exposure is linked to asthma development.
  • The specific mechanisms of cockroach allergen-induced allergy are not fully understood.
  • The role of glycans in cockroach allergy requires further investigation.

Purpose of the Study:

  • To investigate the function of cockroach-associated glycans in human basophil regulation.
  • To identify the specific glycan structures involved in cockroach allergy.
  • To explore the role of C-type lectin receptors (CLRs) in mediating glycan interactions.

Main Methods:

  • Characterization of N-linked glycans from cockroach allergen Bla g 2 using MALDI-TOF mass spectrometry.
  • Assessment of IgE binding and histamine release from basophils.
  • Examination of IL-4 production and CLR expression (DC-SIGN, DCIR, MRC1, dectin-1) in human basophils.
  • Investigation of the role of DCIR in Bla g 2 uptake and basophil activation.

Main Results:

  • Cockroach allergen Bla g 2 possesses complex N-glycans.
  • Deglycosylated Bla g 2 showed reduced IgE binding and histamine release.
  • Cockroach-derived N-glycans inhibited histamine release and IL-4 production.
  • Human basophils express DC-SIGN and DCIR; DCIR mediates Bla g 2 uptake and binding.

Conclusions:

  • Cockroach allergen-associated glycans have a significant, previously unrecognized role in allergic immune responses.
  • DCIR is implicated in regulating basophil function in response to cockroach allergens.
  • Understanding these glycan-basophil interactions may offer new therapeutic targets for cockroach allergy and asthma.