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Related Experiment Videos

Does copper (II) ethylenediaminetetraacetate disproportionate superoxide?

W F Beyer1, I Fridovich

  • 1Department of Biochemistry, Duke University Medical Center, Durham, North Carolina 27710.

Analytical Biochemistry
|August 15, 1988
PubMed
Summary

The Cu(II)EDTA complex does not catalyze superoxide dismutation (O-2), contrary to previous reports. This finding impacts assays for superoxide dismutase enzymes.

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Area of Science:

  • Biochemistry
  • Enzymology
  • Biophysical Chemistry

Background:

  • Previous research suggested Cu(II)EDTA catalyzes superoxide dismutation (O-2).
  • This raised concerns about the validity of using EDTA in superoxide dismutase (SOD) assays.
  • The role of Cu(II)EDTA in O-2 dismutation has been a point of contention.

Purpose of the Study:

  • To clarify the catalytic activity of Cu(II)EDTA on O-2 dismutation.
  • To re-evaluate the use of EDTA in SOD assay methodologies.
  • To investigate discrepancies in reported findings regarding Cu(II)EDTA and O-2.

Main Methods:

  • Assaying the catalytic activity of Cu(II)EDTA on O-2 dismutation.
  • Utilizing both cytochrome c and nitroblue tetrazolium assays.

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  • Examining activity at physiological pH values (6.0 and 7.8).

    • Main Results:

    • Cu(II)EDTA was found to be devoid of O-2 dismutation activity at pH 6.0 and 7.8.
    • Both cytochrome c and nitroblue tetrazolium assays yielded negative results for Cu(II)EDTA catalysis.
    • Discrepancies with prior studies were noted and explored.

    Conclusions:

    • The Cu(II)EDTA complex does not catalyze the dismutation of O-2 under the tested conditions.
    • The use of EDTA to suppress adventitious metal ion activity in SOD assays remains sound.
    • Peculiarities of the nitroblue tetrazolium assay may explain conflicting results.