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Cloning and characterization of a genomic probe for malignant catarrhal fever virus
L M Shih1, J M Irving, Y C Zee
1Department of Veterinary Microbiology and Immunology, School of Veterinary Medicine, University of California, Davis 95616.
Abstract:
A genomic probe specific for malignant catarrhal fever (MCF) virus was cloned by using purified viral DNA from MCF-virus strain WCll. Restriction endonuclease analysis of the purified viral DNA was used to identify the cloned viral genomic fragment. Dot blot hybridization by use of the genomic probe (pRP-5) indicated that the probe hybridized specifically with WCll-MCF virus, as well as with one other isolate of MCF-associated herpesvirus. Hybridization also was observed to a non-MCF virus strain of bovine herpesvirus.
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