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Updated: Mar 3, 2026

Modifications of the Langendorff Method for Simultaneous Isolation of Atrial and Ventricular Myocytes from Adult Mice
Published on: May 13, 2021
Effects of diacetyl-liensinine on electrophysiology in rabbit ventricular myocytes
Feng Cao1,2,3, Teng Wang4,5,6, Wenmao Ding4,5,6
1Department of Cardiology, Renmin Hospital of Wuhan University, Wuhan, 430060, People's Republic of China. cunjiesun1104@sina.com.
Background:
Diacetyl-liensinine is a chemosynthetic derivative of liensinine, extracted from the seed embryo of Nelumbo nucifera Gaertn, in China. It has been found to have extensive anti- arrhythmic actions. The present study was designed to investigate the effects of diacetyl-liensinine on electro- physiology of myocytes.
Methods:
We exposed rabbit ventricular myocytes to diacetyl-liensinine using standard whole-cell patch-clamp technique and measured the action potential, L-type calcium current (I Ca-L), delayed rectifier potassium current (I K), transient outward potassium current (I to) and inward rectifier potassium current (I K1).
Results:
Our results showed that diacetyl-liensinine significantly prolonged action potential duration at 50 and 90% repolarization (APD50, APD90), at 10 and 30 μM, while shortened APD50 and APD90 at 100 μM. In addition, diacetyl-liensinine inhibited the ICa-L, IK, I to and IK1 in a concentration-dependent manner.
Conclusions:
The results suggest that diacetyl-liensinine might be a potential anti-arrhythmic agent.
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