Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Antimicrobial Proteins01:23

Antimicrobial Proteins

14.9K
Antimicrobial proteins are important components of the immune system. They aid the body in combating pathogens by either killing them directly or hindering their replication processes. Four main types of antimicrobial substances are interferons, the complement system, iron-binding proteins, and antimicrobial proteins.
Interferons
Interferons (IFNs) are proteins produced by lymphocytes, macrophages, and fibroblasts infected with viruses. While IFNs cannot prevent viruses from entering and...
14.9K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

1.4K
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
1.4K
Surface Membrane Barriers01:18

Surface Membrane Barriers

3.1K
The skin and mucous membranes serve as the primary line of defense against pathogens by providing both physical and chemical protection. These barriers are essential in preventing the entry and establishment of microbes, thereby maintaining the integrity of the host.
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
3.1K
Biological Methods for Microbial Control01:28

Biological Methods for Microbial Control

1.0K
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
1.0K
Gene Regulation in Microbial Communities: Quorum Sensing01:28

Gene Regulation in Microbial Communities: Quorum Sensing

779
Quorum sensing is a mechanism of bacterial communication that enables coordinated gene expression in response to changes in population density. This facilitates collective behaviors that enhance survival, resource acquisition, and ecological adaptation. This process relies on small signaling molecules called autoinducers that accumulate as bacterial populations grow. When a critical threshold concentration of autoinducers is reached, bacterial cells collectively modify gene expression,...
779

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chromium- and Monolayer Graphene-Based Coating for THz-Assisted Atom Probe Tomography of Sol-Gel Silica Specimens.

Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·2026
Same author

Green Descaling of Gypsum Deposits in Industrial Pipelines: A Chelant-Free Approach.

ACS omega·2026
Same author

Occupational particle exposure and diabetes: a cohort study in Swedish construction workers.

International archives of occupational and environmental health·2026
Same author

Antibacterial Activity of Solid-Supported Gold Nanorods Combined with Antimicrobial Peptides.

ACS applied bio materials·2026
Same author

Integrated FHIT and IDH2 biomarker profiling predicts lethal sensitivity to DCPS inhibition in Acute Myeloid Leukemia and Myelodysplastic syndrome.

Discover oncology·2026
Same author

Monte Carlo calculations of the simulated radiation dose distribution from a dental bitewing X-ray exposure and the usefulness of thyroid protection.

Radiation protection dosimetry·2026

Related Experiment Video

Updated: Mar 3, 2026

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
10:13

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization

Published on: August 11, 2018

12.8K

Cubosomes post-loaded with antimicrobial peptides: characterization, bactericidal effect and proteolytic stability.

Lukas Boge1, Anita Umerska2, Nada Matougui2

  • 1RISE Research Institutes of Sweden, Drottning Kristinas väg 45 Box 5607 Stockholm SE 11486, Sweden; Department of Chemistry and Chemical Engineering, Applied Chemistry, Chalmers University of Technology, Kemigården 4 Göteborg SE-41296, Sweden.

International Journal of Pharmaceutics
|May 7, 2017
PubMed
Summary

Cubosomes effectively deliver antimicrobial peptides (AMPs) as novel antibiotics. These peptide-loaded cubosomes show varied antimicrobial effects and protect LL-37 from degradation.

Keywords:
AmpAntimicrobial peptideCubosomeGlycerol monooleateLiquid crystalline nanoparticleProteolysis

More Related Videos

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

9.3K
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

13.1K

Related Experiment Videos

Last Updated: Mar 3, 2026

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization
10:13

Production and Visualization of Bacterial Spheroplasts and Protoplasts to Characterize Antimicrobial Peptide Localization

Published on: August 11, 2018

12.8K
A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles
09:57

A Facile and Efficient Approach for the Production of Reversible Disulfide Cross-linked Micelles

Published on: December 23, 2016

9.3K
Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids
11:56

Antimicrobial Peptides Produced by Selective Pressure Incorporation of Non-canonical Amino Acids

Published on: May 4, 2018

13.1K

Area of Science:

  • Biomaterials Science
  • Drug Delivery Systems
  • Antimicrobial Research

Background:

  • Novel antibiotics are crucial due to rising antimicrobial resistance.
  • Antimicrobial peptides (AMPs) show promise but face challenges like degradation.
  • Cubosomes offer a unique nanostructure for drug encapsulation and delivery.

Purpose of the Study:

  • To evaluate cubosomes as drug delivery vehicles for three antimicrobial peptides (AMPs): AP114, DPK-060, and LL-37.
  • To investigate the association, structural impact, antimicrobial efficacy, and proteolytic protection of AMP-loaded cubosomes.
  • To explore the potential of cubosomes in enhancing AMP stability and activity.

Main Methods:

  • High-performance liquid chromatography (HPLC), ζ-potential, and circular dichroism for peptide association.
  • Small-angle X-ray scattering (SAXS) and cryogenic transmission electron microscopy (cryo-TEM) for structural analysis.
  • Minimum inhibitory concentration (MIC), time-kill assays, and radial diffusion assays for antimicrobial activity and proteolytic protection.

Main Results:

  • Varying association efficiencies of AMPs onto cubosomes were observed, with LL-37 showing the highest (>60%).
  • AP114-loaded cubosomes retained antimicrobial effect; LL-37 loaded cubosomes showed reduced broad-spectrum activity but retained Gram-negative efficacy.
  • DPK-060 loaded cubosomes demonstrated enhanced activity against S. aureus and E. coli. Cubosomes protected LL-37 from elastase degradation, improving its bactericidal effect post-proteolysis.

Conclusions:

  • Cubosomes can be effectively utilized for the delivery of antimicrobial peptides, with varying outcomes depending on the specific peptide.
  • The cubosome formulation offers proteolytic protection, particularly for LL-37, enhancing its stability and therapeutic potential.
  • Further research into peptide-cubosome interactions is warranted to optimize delivery systems for enhanced antimicrobial therapies.