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Superoxide anion measurement by sulfonated phenyl isothiocyanate cytochrome c
F Morel1, A C Dianoux, P V Vignais
1Département de Recherche Fondamentale, Centre d'Etudes Nucléaires, Grenoble, France.
Biochemical and Biophysical Research Communications
|November 15, 1988
Abstract:
Sulfonated phenyl isothiocyanate cytochrome c is suggested as a new scavenger for superoxide anion. The efficiency of the modified cytochrome c in measurements is compared with that of phenyl isothiocyanate cytochrome c and acetylated cytochrome c. Sulfonated phenyl isothiocyanate cytochrome c is water and salt soluble. Autooxidability of the pigment is not observed. The primary advantage of sulfonate phenyl isothiocyanate cytochrome c is that it appears to be specifically reduced by O2 radicals without interferences by other reactions in complex biological systems.