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Two linear plasmid-like DNA elements simultaneously maintained in Pleurotus ostreatus.

Y Yui1, Y Katayose, K Shishido

  • 1Department of Life Science, Faculty of Science, Tokyo Institute of Technology, Yokohama, Japan.

Biochimica Et Biophysica Acta
|November 10, 1988
PubMed
Summary
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Researchers discovered two novel linear plasmid-like DNA elements, pLP01 and pLP02, in the edible mushroom Pleurotus ostreatus. These elements possess distinct protein-bound ends and show no genetic similarity to each other or the fungus

Area of Science:

  • Mycology
  • Molecular Biology
  • Genetics

Background:

  • Mitochondrial DNA in fungi can harbor extrachromosomal elements.
  • Linear plasmids with protein-bound ends are known in some eukaryotes.
  • The genetic makeup of Pleurotus ostreatus mitochondria is not fully characterized.

Purpose of the Study:

  • To isolate and characterize novel DNA elements from Pleurotus ostreatus.
  • To investigate the nature and relationship of these elements to the host genome.

Main Methods:

  • Isolation of DNA from mitochondrial preparations of Pleurotus ostreatus.
  • Characterization of DNA elements using gel electrophoresis and proteinase K digestion.
  • Homology studies using Southern blotting and DNA sequencing (implied).

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Main Results:

  • Two linear plasmid-like DNA elements, pLP01 (10.0 kb) and pLP02 (9.4 kb), were identified.
  • Both elements exhibit 5' end protein-binding, with distinct protein characteristics.
  • pLP01 and pLP02 show no sequence homology to each other or to nuclear/mitochondrial genomic DNA.

Conclusions:

  • Pleurotus ostreatus harbors unique linear mitochondrial plasmids.
  • The terminal proteins on pLP01 and pLP02 are distinct, suggesting different origins or functions.
  • These plasmid-like DNAs represent novel genetic components within the P. ostreatus mitochondrial system.